China
Africa
Explore chapters and articles related to this topic
Terpenoids: The Biological Key Molecules
Published in Dijendra Nath Roy, Terpenoids Against Human Diseases, 2019
Moumita Majumdar, Dijendra Nath Roy
Apoptosis or type-1 programmed cell death is initiated by the activation of death receptor (DR) signalling through the binding of the Fas receptor (FasR), TNF receptor (TNFR), DR3, DR4 and DR5 with their respective ligands, resulting in conformational change. After oligomerisation of the receptor via ligands, specialized adaptor proteins activate the caspase cascades. Trimerisation of Fas by binding with FasI recruits adaptor protein Fas-associated death domain (FADD), which activates caspase-8. After oligomerisation, caspase-8 initiates the signalling that stimulates apoptosis via two parallel pathways. In the absence of the caspase cascade, death receptors activate another alternative signalling system designated as necroptosis via formation of the IIb complex. As the largest group of natural products, plant terpenoids have a promising effect on death domain signalling.
A new Sr(II)-based coordination polymer: structural insight and anti-gastric cancer activity
Published in Inorganic and Nano-Metal Chemistry, 2020
Xiaoxia Lu, Xuanxuan Wang, Xun Gong, Cuiyun Wang, Hailing Xu
As reported, the cancer cell apoptosis is usually caused by the activation of the caspase-3 and caspase-8. After activated, the caspase-3 and caspase-8 could be cleaved, which could be detected by western blot and used as the indicator of cell apoptosis.[19] Thus, in this experiment, the western blot assay was carried out to detect the protein level of cleaved caspase-3 and caspase-8 in MKN45 gastric cancer cells after nano complex treatment. The western blot results in Figure 5a suggested that nano complex obviously increased the relative level of cleaved caspse-3 and caspase-8 and showed a dose relationship with the nano complex concentration, which further convinced the apoptosis induced by nano complex in MKN45 cells. Figure 5b is the relative protein expression calculated with Quantity One version 4.1.0. The above experiment convinced that nano complex induced the cell apoptosis via triggering the activation of caspase-3 and caspase-8.