China
Africa
Explore chapters and articles related to this topic
Chemical Equilibrium
Published in Armen S. Casparian, Gergely Sirokman, Ann O. Omollo, Rapid Review of Chemistry for the Life Sciences and Engineering, 2021
Armen S. Casparian, Gergely Sirokman, Ann O. Omollo
To determine the pH of a buffer solution, the Henderson–Hasselbalch equation may be used. In the case of a weak acid (e.g., CH3COOH) and its conjugate base salt (e.g., NaCH3COO): pH=pKa+log[conjugatebase][weak acid]
Capillary Electrophoresis
Published in Grinberg Nelu, Rodriguez Sonia, Ewing’s Analytical Instrumentation Handbook, Fourth Edition, 2019
The buffer solution should resist pH change on dilution and addition of small amounts of acids and bases. Concentrated buffer solutions do this well but can be too conductive for use in CE. The buffering capacity of a weak acid or weak base is limited to ±1 pH unit of its pKa. CE operation outside of this range requires frequent buffer replacement to avoid pH changes [11]. Aromatic buffer constituents such as phthalates should be avoided, if possible, because of their strong UV chromophore. In addition, strongly absorbing components such as carrier ampholytes prevent the use of the low UV detector wavelengths.
Marine sediment derived bacteria Enterobacter asburiae ES1 and Enterobacter sp. Kamsi produce laccase with high dephenolisation potentials
Published in Preparative Biochemistry & Biotechnology, 2021
Chiedu E. Edoamodu, Uchechukwu U. Nwodo
Determination of optimum pH for laccase activity was performed at various buffer (pH 3.0–11.0) values prepared using 100 mM concentration. The buffer solution applied were adjusted using sodium citrate (pH 3.0–5.0), potassium phosphate (pH 6.0–7.0), Tris HCl (pH 8.0), and glycine-NaOH (pH 9.0–11.0). Also, the effect of varied temperature (20–100 °C) conditions on laccase activity was determined by adding the purified laccase to the preheated buffer solution the laccase solution at the varied temperatures conditions. Laccase activity was assayed following the standard protocol.[30]