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Imaging Fibrillar Collagen with Optical Microscopy
Published in Jiro Nagatomi, Eno Essien Ebong, Mechanobiology Handbook, 2018
Tong Ye, Peng Chen, Yang Li, Xun Chen
The staining technique in histology is routinely used for observing collagen fibers with colors. The most common histological staining techniques for identification of collagen are Movat's pentachrome and Masson's trichrome stain (Bradbury 1973; Locquin and Langeron 1983); thin sections are need to perform staining and imaging. Picrosirius red (Locquin and Langeron 1983) is commonly used as both a colored dye and fluorescent probe for collagen, and provides a sensitive and efficient tool for visualizing the three-dimensional architecture of collagen in tissue (Wu et al. 2006). Picrosirius red is a linear polar molecule with a high binding affinity to collagen molecules, and thus enhances the birefringence of collagen fibrils (see Section 6.3.3). Fluorescein has recently been found to be able to bind collagen for three-dimensional visualization of collagen networks in thick tissue sections (Staszyk and Gasse 2004).
Attenuation of streptozotocin induced high fat diet exacerbated dyslipidemia mediated hepatic and aortic injuries in male pigs by camel milk
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Hadiza Bello Rilwan, Sunday Samuel Adebisi, James Abrak Timbuak, Sunday Blessing Oladele, Aliyu Muhammad, Wusa Makena, Adamu Abubakar Sadeeq
Masson’s trichrome staining was done with a ready-to-use kit (Trichrome Stain Kit). In a nutshell, the tissue slices were sliced into 5 m thick pieces and put on typical microscopy slides. After deparaffinisation and rehydration, the slides were submerged in Bouin’s solution for 15 minutes at 56°C. The slides were then rinsed with tap water for 5 minutes. The slices were then stained with Weigert’s hematoxylin for 5 minutes before being washed in tap water for 5 minutes and rinsed in distilled water. The slides were then stained for 5 minutes with Biebrich scarlet-acid fuchsin, washed in distilled water, incubated in phosphotungstic-phosphomolybdic acid for 5 minutes, colored with aniline blue for 5 minutes, and fixed in 1% acetic acid for 2 minutes. Finally, the slides were rinsed in distilled water, dehydrated, and mounted. Finally, the slides were dehydrated and mounted after being rinsed in distilled water [31]. The tissues were then viewed under a light microscope, and photomicrographs were taken with a digital microscope camera (AmScope MD 900) at a magnification of ×250.
Histological and immunohistochemical study of the effect of liraglutide in experimental model of non-alcoholic fatty liver disease
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Mai Salah Nour, Zeinab Abd El-Hay Sakara, Nawal Awad Hasanin, Shereen Mohamed Hamed
Specimens were preserved in 10% buffered formalin for 1 day. They were prepared for paraffin sections by gradually dehydration using ascending grades of alcohol concentrations, clearing in xylene and being embedded in soft and then hard paraffin wax. Using a microtome, sections were cut at a thickness of 5–6 µm and prepared for histological and immunohistochemical study of the structural changes in NAFLD. Tissue sections were stained with; Hematoxylin and Eosin (H&E) [15] for the routine histological examination, Mallory’s Trichrome Stain [16] for the demonstration of collagen and Oil Red O stain [17] for identification of lipids in frozen sections.
Electrospun polysaccharide scaffolds: wound healing and stem cell differentiation
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Preethi G. U., Unnikrishnan B. S., Sreekutty J., Archana M. G., Deepa Mohan, Raveendran Pillai K., Sreelekha T. T.
After specific time points, mice were euthanized and tissue from the wound was removed and fixed in 10% formaldehyde followed by staining for histology and collagen distribution using hematoxylin and eosin (H&E) and Masson’s trichrome stain. Olympus BX45 Motorized System Microscope with a CCD camera (Nikon, Japan) was used to obtain all images.