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Brain Targeted Drug Delivery Systems
Published in Ambikanandan Misra, Aliasgar Shahiwala, In-Vitro and In-Vivo Tools in Drug Delivery Research for Optimum Clinical Outcomes, 2018
Manisha Lalan, Rohan Lalani, Vivek Patel, Ambikanandan Misra
Subcellular systems have been useful in study of signaling pathways and receptor-mediated signal transduction studies. Isolated mitochondria are separated from the whole brain and they may be subclassified as synaptosomal fractions, heavy and light mitochondria, and free mitochondria from nonsynaptic origin. They have been used extensively for study of the interference of several neurotoxicants, including drugs, on mitochondrial bioenergetics. A synaptosome model is another popular subcellular model derived from neurons by brain tissue homogenization and functions as small anucleated cells that retain neuronal vesicles, enzymes, and active ion transport systems. This simple preparation is a tool to study metabolic pathways, ion movements, neurotransmitters’ synthesis, storage and release, and insults to mitochondria, as well as a way to replicate the mitochondrial deficits found in neurodegenerative diseases (Pereira et al. 2009; Barbosa et al. 2015).
Essential variables for air quality estimation
Published in International Journal of Digital Earth, 2020
Andrii Shelestov, Andrii Kolotii, Tatiana Borisova, Olena Turos, Gennadi Milinevsky, Igor Gomilko, Tatyana Bulanay, Oleg Fedorov, Leonid Shumilo, Liudmyla Pidgorodetska, Liudmyla Kolos, Arsenii Borysov, Natalia Pozdnyakova, Alexander Chunikhin, Marina Dudarenko, Arina Petrosian, Vassyl Danylevsky, Natalia Miatselskaya, Vasyl Choliy
The experiments dedicated to study the influence of PM on the human nervous system are based on the following methods: Isolation of rat brain nerve terminals (synaptosomes) (Borisova 2016; Borisova and Borysov 2016). The synaptosome preparations were obtained by differential and density gradient centrifugation of brain homogenate according to (Borisova 2014; Borisova et al. 2016).Recording of the plasma membrane potential (Em) of rat brain nerve terminals. Measurements were performed using a potentiometric fluorescent dye rhodamine 6G (0.5 µM) at 528 and 551 nm of excitation and emission wavelengths, respectively, with a spectrofluorimeter Hitachi MPF-4 (Borisova 2013; Borisova 2019).Analysis of water-suspended wood smoke aerosol preparations by dynamic light scattering (Borisova 2013). Water-suspended wood smoke aerosol preparations were analyzed by dynamic light scattering with a laser correlation spectrometer Zetasizer-3 (Malvern Instruments, UK), equipped with He–Ne laser LGN-111 (P = 25 mW, λ = 633 nm). Registration and statistical processing of laser scattered from water (n = 1.33) suspensions of particles were performed repeatedly for 60 s at +22°C with scattering angle 90°. Data were processed with computer software service PCS-Size mode v1.61. The laser correlation spectrometer was equipped with multi-computing correlator type 7032 ce.