China
Africa
Explore chapters and articles related to this topic
Articular Cartilage Development
Published in Kyriacos A. Athanasiou, Eric M. Darling, Grayson D. DuRaine, Jerry C. Hu, A. Hari Reddi, Articular Cartilage, 2017
Kyriacos A. Athanasiou, Eric M. Darling, Grayson D. DuRaine, Jerry C. Hu, A. Hari Reddi
Sox5, Sox6, and Sox9 are members of the Sox (sex-determining region Y-box) protein family and the High-Mobility Group (HMG) super family of DNA binding proteins, which are characterized by sequence homology to the HMG domain of the sex-determining region on the Y chromosome. Sox9 is the master regulator of chondrocyte differentiation and is coexpressed with Sox5 (as L-Sox5) and Sox6 (Akiyama et al. 2002; Akiyama 2008).
Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon
Published in Nano Reviews & Experiments, 2018
Abbas Zakeri, Mohammad Amin Jadidi Kouhbanani, Nasrin Beheshtkhoo, Vahid Beigi, Seyyed Mojtaba Mousavi, Seyyed Ali Reza Hashemi, Ayoob Karimi Zade, Ali Mohammad Amani, Amir Savardashtaki, Esmail Mirzaei, Sara Jahandideh, Ahmad Movahedpour
In 2017, drug delivery and the effectiveness of BMPPEI complex-conjugated with the foreign DNAs (BPDs) in promoting the testes-mediated gene transfer (TMGT) in mice were studied and the BPDs were compared with the liposome-conjugated foreign DNAs. The results showed that BPD clusters successfully reached cytoplasm and spermatogenesis cell nucleus through the injection test and expressed in the testicle of the transgene founder mice [34]. In 2017, Shih and Venault conducted a study on zwitterionic-shielded carrier with a reversible pH modulation for gene delivery. They exhibited a good design of core-shell polyplexes, which showed PEI as the core and zwitterionic poly(acrylic acid)-block-poly(sulfobetaine methacrylate), ((PAA-b-PSBMA) diblock copolymer) as the shell. Gel retardation tests and displacement of ethidium bromide were used to determine the PEI/DNA or PDMAEMA/DNA complexation. In neutral pH, polymer acts as a protective shell of the complex. Therefore, the results of this study provide a pathway for improving the efficiency and non-toxic properties of pH-sensitive gene carriers [23]. In 2017, Park et al., constructed PLGA nanoparticles coated with Polycistronic SOX5, SOX6 and SOX9 genes for mesenchymal human stem cell chondrogenesis. This multivariate plasmid was complexed with PEI polycationic polymer and poly (lactic-co-glycolic) acid (PLGA) nanoparticles were coated with complex genes (SOX5, SOX6, and SOX9) presented more condrogenic differences than each single gene transfection method [35]. In 2017, Guan et al., study on a pH-responsive detachable polyethyleneglycol (PEG) shielding strategy for gene delivery system in cancer therapy. The primary study endpoints are safety, tolerability and immunogenicity of DNA-PEI vaccination [36]. In 2016, Yang et al., found that the binding of bacterial magnetic particles to PEI increased the gene delivery to breast cells. They reported a method for constructing BMPs-PEI/DNA complexes, which resulted in improved transmission efficiency, reduced cytotoxicity and shorter working time than current methods for formation and transfection of both complexes [37]. In 2016, Santos et al., reported a flash nanocomplexation (FNC) method that continuously produces LPEI DNA/plasmid nanoparticles with a narrow size distribution using a confined impinging jet device. This method involves a combination of negatively charged DNA with positively charged LPEI in fast, high dynamic and homogeneous conditions, which produces complexes of poly-electrolytic nanoparticles with a narrow distribution of particle size and shape [29] (Figure 4).