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Differential Protein Expression Following JP-8 Jet Fuel Exposure
Published in Mark L. Witten, Errol Zeiger, Glenn D. Ritchie, Jet Fuel Toxicology, 2010
Frank A. Witzmann, Mark L. Witten
As in the lung proteome experiments described previously, 2-DE of cytoplasmic proteins prepared from whole kidney homogenates of Swiss-Webster mice exposed to 1,000 mg/m3 aerosolized JP-8 for 1 hour/day for 7 days [16] revealed nearly 1,000 proteins that were matched across 12 individual patterns (n = 6). When between-group statistics were calculated and protein expression compared [74], it was apparent that JP-8 exposure had significantly altered (p < 0.05) the expression of 56 proteins spots (21 up-regulated and 35 down-regulated); 26 of these protein spots (representing 22 individual proteins) were identified by peptide mass fingerprinting, and these proteins, listed in Table 5.3, can be categorized functionally as follows: (1) ultrastructural abnormalities, (2) altered protein processing, (3) metabolic effects, and (4) stress protein/detoxification system response.
Plant responses to per- and polyfluoroalkyl substances (PFAS): a molecular perspective
Published in International Journal of Phytoremediation, 2023
Ayesha Karamat, Rouzbeh Tehrani, Gregory D. Foster, Benoit Van Aken
Besides evidence from enzymatic activities and PFAS metabolites, transcriptomic studies on plant exposed to PFAS have shown activation of genes involved in the xenobiotic metabolism. Using A. thaliana exposed to PFOA, Fan et al. (2020) reported an increase in the level of expression of several genes potentially involved in the transport of PFAS (41 and 29 genes in exposed shoots and roots, respectively), including ABC transporters, drug transmembrane transporters, and ion and alternative active transmembrane transporters (e.g.,DTX1 (DETOXIFICATION 1), ABCB11, and ABCC3), suggesting involvement of phase III enzymes on the transport of PFAS in plant tissues. Further examination of transcriptomic data made available by Fan et al. (2020) revealed overexpression of a range of phase I, II, and III enzymes potentially involved in the response of A. thaliana to PFAS, including GSTs, CYPs, and ABC transporter proteins. In their study on the effects of the short-chain PFBA on soybean plants, Omagamre et al. (2022) similarly reported upregulation of multiple phase I, II, and III genes, encoding CYPs, ABC transporter proteins, pleiotropic drug resistance proteins, protein DETOXIFICATION, and pathogenesis-related proteins.