China 
Africa 
Explore chapters and articles related to this topic
MOF-based Electrochemical Sensors for Biological Macromolecule Sensing
Published in Ram K. Gupta, Tahir Rasheed, Tuan Anh Nguyen, Muhammad Bilal, Metal-Organic Frameworks-Based Hybrid Materials for Environmental Sensing and Monitoring, 2022
Peihong Tong, Yongjuan Meng, Jianping Li
In addition, the increase of the concentration of N-terminal pro–B-type natriuretic peptide (NT-proBNP) is considered to be an objective indicator for the diagnosis of heart failure (HF). Li et al. constructed a label-free electrochemiluminescence immunosensor using a cerium metal-organic framework/graphite phase carbon nitride nanosheet/Au nanoparticle composite material (Ce-MOF@g-C3N4/Au) for sensitive detection of NT-proBNP [50]. Ce-MOF has a large specific surface area and excellent adsorption performance, which not only improves the ECL efficiency of g-C3N4, but also significantly improves the interface stability of g-C3N4. In addition, a large number of Au NPs are loaded on the surface of Ce-MOF@g-C3N4, which not only improves the conductivity of Ce-MOF@g-C3N4, but also facilitates the immobilization of NT-proBNP antibody and obtains an excellent immunosensor platform. The ECL immunosensor showed excellent stability and specificity. Polypeptide glycosylation is closely related to many biological and cellular functions, including signal transduction, cell proliferation, and differentiation. At present, mass spectrometry (MS) is an important and effective method for the identification of glycopeptides. Ma et al. loaded Au nanoparticles in-situ on MIL-101(NH2), and then immobilized L-cysteine (Cys) on Au nanoparticles by Au-S bond to obtain Cys-functionalized MIL-101(NH2) complex (MIL-101(NH2)@Au-Cys) [51]. Due to the large specific surface area and ultra-high hydrophilicity of the nanocomposite, it exhibits excellent performance for the adsorption and enrichment of glycopeptides. N-glycopeptide in HeLa cell lysate was enriched by MIL-101(NH2)@Au-Cys, and then determined by MS. The results are shown in Figure 24.6. MIL-101(NH2)@Au-Cys has the characteristics of short incubation time, strong binding ability, good selectivity, high recovery (more than 80%), and low detection limit (1 fM) for glycopeptide enrichment. This work not only opens up a new way for the rapid functionalization of MOFs but also provides a new idea for the design of new sample-enrichment materials, especially in the correlation analysis of glycopeptideomics, which has a good application prospect.
Potential adverse cardiac remodelling in highly trained athletes: still unknown clinical significance
Published in European Journal of Sport Science, 2018
Luigi Gabrielli, Marta Sitges, Mario Chiong, Jorge Jalil, María Ocaranza, Silvana Llevaneras, Sebastian Herrera, Rodrigo Fernandez, Rodrigo Saavedra, Fernando Yañez, Luis Vergara, Alexis Diaz, Sergio Lavandero, Pablo Castro
B-type natriuretic peptides are secreted in response to cardiomyocyte stress produced by pressure or volume overload. Elevated levels indicate cardiac dysfunction and its levels are used as an important diagnostic tool, for monitoring therapy and for risk stratification in patients with cardiovascular disease. B-type natriuretic peptides levels are similar in endurance athletes compared to matched controls at rest, but increase several fold after exercise (Ohba et al., 2001). Some authors attribute these increases to subclinical myocardial injury (Mant, Al-Mohammad, Swain, Laramee, & Guideline Development, 2011). However, there is no clear relationship between B-type natriuretic peptides level and cardiac size and function in athletes. Moreover, collapsed marathon runners showed lower levels compared with asymptomatic runners, suggesting that this exercise-induced elevation is not pathologic (Siegel et al., 2008). In conclusion, the clinical implications of these observations are unknown.