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Liposome-Scaffold Systems for Drug Delivery
Published in Vladimir Torchilin, Handbook of Materials for Nanomedicine, 2020
Similarly to other hydrogels, stimuli-responsive composites have been generated with collagen-complexed liposome systems. Lopez-Noriega [57] et al. developed a temperature-sensitive composite by incorporating thermoresponsive liposomes within collagen-hydroxyapatite scaffolds. In this example, the scaffolds were functionalized with sulfhydryl groups, and subsequently complexed to DPPC-MSPC-DSPE-PEG2000-maleimide liposomes encapsulating the parathyroid hormone-related protein (PTHrP 107–111). In vitro, this system exhibited pro-osteogenic and anticatabolic effects on MC3T3-E1 bone cells. Hyperthermic pulses controlled cargo release, which otherwise occurs at a steady rate (50% release after 14 days). Alkaline phosphatase activity, alongside expression of OPN and OCN genes, demonstrated effectiveness of the applied pulses.
Bioresorbable Hybrid Membranes for Bone Regeneration
Published in Severian Dumitriu, Valentin Popa, Polymeric Biomaterials, 2020
Osteoblast-like cells (MC3T3-E1 cells) from rat calvaria were seeded onto SiPVC+ or PVC+ sterilized using ethylene oxide gas in a 24-well plate at a density of 60,000 cells per well. Alpha-modified minimum essential medium containing 10% fetal bovine serum (FBS) was used as the culture medium and changed every 2 days. Cells were cultured at 37°C in a humidified incubator with 5% CO2, harvested after 1, 3, and 5 days using 0.1% actinase in PBS, and counted by a hemocytometer. Figure 6.2 shows the cellular numbers of MC3T3-E1 cells on SiPVC+ and PVC+ in a 24-well plate after incubation. The cells satisfactorily proliferated on both sample surfaces, and the cellular numbers on SiPVC+ were significantly higher than that on PVC+.
Biomedical Applications of Organic Conducting Polymers
Published in John R. Reynolds, Barry C. Thompson, Terje A. Skotheim, Conjugated Polymers, 2019
Alexander R. Harris, Paul J. Molino, Caiyun Wang, Gordon G. Wallace, Zhilian Yue
Formation of highly porous structures has been achieved through an ice templating method [167]. A dispersion of PEDOT–PSS and 3-glycidoxypropyltrimethoxysilane crosslinker was frozen and then lyophilised before annealing at 140˚C for 2 hours. Median pore diameter was homogeneous and varied from ~50–100 µm depending on experimental conditions and measurement technique. These scaffolds were then able to support differentiation of osteogenic precursor cells (MC3T3–E1) osteoblasts for potential bone tissue engineering. Another porous structure formed from a PEDOT–PSS sol–gel composite enabled human mesenchymal stem cell attachment and proliferation [168].
Comparison of surface properties, cell behaviors, bone regeneration and osseointegration between nano tantalum/PEEK composite and nano silicon nitride/PEEK composite
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Gangfeng Hu, Yuan Zhu, Fangqi Xu, Jiakuan Ye, Jie Guan, Yiqian Jiang, Meijuan Di, Zhennan Li, Hong Guan, Xiaocong Yao
The cellular response to the samples (PEEK, SPC, TPC) was determined by using mouse osteoblast-like MC3T3-E1 cells (American Type Culture Collection, Chinese Academy of Sciences, China) in vitro. The samples were sterilized by UV radiation, which were then placed into 12-well plates. The MC3T3-E1 cells (2 × 104 cells per well) were cultured with 5% CO2 at 37 °C in α-minimum essential medium (Wisent Co., Canada) with 10% (v/v) fetal bovine serum (FBS, Wisent Co., Canada) and 1% (v/v) penicillin/streptomycin (Procell Life Science & Technology Co., China), while the medium was replaced every 3 days. Before cells achieved confluence, 0.25% trypsin EDTA (Sigma-Aldrich Co., USA) was applied to collect cells. Before seeding on samples, the cells were then resuspended in fresh medium and the cells at population of 3–5 passages were performed.
Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: In-vitro evaluations
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Muhammad Umar Aslam Khan, Muhammad Rizwan, Saiful Izwan Abd Razak, Anwarul Hassan, Tahir Rasheed, Muhammad Bilal
In this study, we have developed a co-dispersed nanosystem of Fe3O4/GO and used bacterial cellulose to synthesize polymeric nanocomposite materials from the co-dispersed nanosystem nano-hydroxyapatite (nHAp). Porous scaffolds were fabricated from polymeric nanocomposite material using a freeze-drying technique and coated with collagen to improve bioactivity. FT-IR, XRD, SEM, and UTM were used to conduct structural, surface morphology, and mechanical studies. Wetting, swelling, and degradation tests were carried out, and antibacterial activities were conducted against Gram + ive and Gram -ive bacteria. The biological properties were tested on the MC3T3-E1 cell line from a mouse pre-osteoblast. The porous scaffolds that result could be useful biomaterials for bone tissue engineering.
3D printing silk-gelatin-propanediol scaffold with enhanced osteogenesis properties through p-Smad1/5/8 activated Runx2 pathway
Published in Journal of Biomaterials Science, Polymer Edition, 2021
Caiyun Liu, Zhenzu Bai, Jiayu Lin, Kai Jiang, Shan Huang, Weijia Zheng, Ruifen Chen, Yang Xiang, Xiaoyan Wang, Long Liu
MC3T3-E1 pre-osteoblasts were used in vitro to study osteoblast adhesion, proliferation, differentiation, and mineralization to determine which type of scaffold is best suited for bone tissue regeneration. In addition, in order to investigate the major signaling pathways regulating osteogenic differentiation of osteoblasts cultured on scaffolds, we choose two classic pathways, Smad and Erk, which play important roles in the proliferation, differentiation, and mineralization of osteoblasts. It is reported that Phosphorylated Smad and Phosphorylated Erk can activate the expression of Runx2 to promote the expression of osteoblast marker genes, enhancing the cell differentiation and mineralization [18, 19].