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Graphene-Based Optical Biosensors and Imaging
Published in Li Jun, Wu Nianqiang, Biosensors Based on Nanomaterials and Nanodevices, 2017
Tang Zhiwen, He Shijiang, Pei Hao, Du Dan, Fan Chunhai, Lin Yuehe
Park and colleagues have constructed a GO-based immunosensor via the quenching of the innate fluorescence of GO in the presence of the peroxidase-catalyzed polymerization product.22 In this study, the interleukin-5 (IL-5), a key cytokine associated with asthma pathology and eosinophilia, is used as the analyte model. After depositing the GO onto the amino-modified glass surface, one IL-5 antibody is immobilized as the capture antibody. The captured IL-5 is recognized and bound with another peroxidase-linked IL-5 antibody. In the presence of peroxide, the 3,3′-diaminobenzidine (DAB) is catalyzed to form the polymerization product, which is adsorbed on the GO surface, and effectively quenches the fluorescence of GO. The GO-based immunoassay has demonstrated high specificity for IL-5 among other cytokines and is not affected by human serum proteins. The detection limit of IL-5 is about 5 pg/mL. This GO-based immunoassay platform can be readily adapted for the detection of other protein targets, by changing antibodies.
Eosinophilic pneumonia induced by drugs
Published in Philippe Camus, Edward C Rosenow, Drug-induced and Iatrogenic Respiratory Disease, 2010
In most cases of drug-induced eosinophilia, the pivotal cell involved in the initiation of the host response is the T-lymphocyte, specifically the Th2 (T-helper type 2) lymphocyte. Interleukin-5 (IL-5) produced by Th2 cells is the main cytokine regulating eosinophil production by the bone marrow (Fig. 23.1). Other cytokines promoting eosinophil production include IL-3 and granulocyte–macrophage colony stimulating factor.3
Comparative electrophysiological evaluation of hippocampal function following repeated inhalation exposures to JP-8, Jet A, JP-5, and the synthetic Fischer Tropsch fuel
Published in Journal of Toxicology and Environmental Health, Part A, 2018
Joyce G. Rohan, Shawn M. McInturf, Molly K. Miklasevich, Chester P. Gut, Michael D. Grimm, James E. Reboulet, William R. Howard, Karen L. Mumy
Approximately 100 µl blood was collected from the lateral tail vein of rats prior to initiation of exposure and within 24 hr following the last day of exposure. The following nine pro-inflammatory cytokines were determined using the Meso Scale Discovery (MSD) multiplex kit and the Sector Imager 2400 instrument: IFN-γ (interferon gamma), IL-1β (interleukin 1β), IL-4 (interleukin 4), IL-5 (interleukin 5), IL-6 (interleukin 6), IL-10 (interleukin 10), IL-13 (interleukin 13), KC/GRO (keratinocyte chemoattractant/ human growth-regulated oncogene chemokines), and TNF-α (tumor necrosis factor alpha). Cytokine levels were measured before and after the 28-d occupational exposure. Normalized data were calculated as the cytokine level (pg/ml) after last day of exposure divided by concentration before the first day of exposure. In Phase 4 (JP-5 exposure), blood was collected at baseline, following the 4th day of treatment, and within 24 hr as well as 2 weeks after completion of exposures.
Mouse pulmonary response to dust from sawing Corian®, a solid-surface composite material
Published in Journal of Toxicology and Environmental Health, Part A, 2019
W. Kyle Mandler, Chaolong Qi, Marlene S. Orandle, Khachatur Sarkisian, Robert R. Mercer, Aleksandr B. Stefaniak, Alycia K. Knepp, Lauren N. Bowers, Lori A. Battelli, Justine Shaffer, Sherri A. Friend, Yong Qian, Jennifer D. Sisler
The first cell-free BALF from 1 and 14 days post-exposure mice was utilized to analyze cytokine and chemokine protein levels as previously described (Mercer et al. 2013) with a V-Plex pro-inflammatory Panel (mouse) kit (Meso Scale Discovery, Gaithersburg, MD) using the manufacturer guidelines. Samples were run in duplicate. The following cytokine/chemokines were determined: interferon gamma (IFN-γ), interleukin-10 (IL-10), interleukin-12 (IL-12p70), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-6 (IL-6), neutrophil-activating protein 3 (KC/GRO), and tumor necrosis factor-alpha (TNFα).