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AI and Chronic Inflammation
Published in Louis J. Catania, AI for Immunology, 2021
A brief summary of this complex theory starts with part of an antibody binding with a specific antigen. Then generated B-cells begin to produce genetically cloned antibodies with unique profiles of idiotypic epitopes (called idiotypes or antigen-binding sites for the cloned antibodies) that increase immunogenic stimulation. This stimulation induces anti-idiotype and anti-anti-idiotype antibodies (called antibody-2 and antibody-3, and beyond) which ultimately suppress continued stimulation by binding with compatible T suppressor cells. This binding produces a regulatory closed-loop suppressor system (or circuit) in the lymphoid system and provides antibodies which can eliminate a persistent antigen, in the case of cancer, the carcinogen or carcinogenic stimulus. These anti-idiotype antibodies have the potential to provide long-lasting immunity as a vaccine for cancer.27 (I told you it was complex! But its potential benefits to our public health and humanity definitely earn it a place in this discussion.)
Preparation monoclonal β-type anti-idiotype antibody of zearalenone and development of green ELISA quantitative detecting technique
Published in Preparative Biochemistry & Biotechnology, 2020
Luhuai Shi, Tao Yu, Miner Luo, Hong Wang
In order to confirm that the anti-idiotype antibody was the one pairing with anti-ZEN mAb, but not the other mouse IgG. The cross-reaction analysis was tested by using indirect ELISA between biotinylated-Ab2β-1D5 and mouse-derived other monoclonal antibodies. Briefly, anti-ZEN mAb, anti-Ractopamine mAb, anti-Plumbum mAb, anti-Chromium mAb and anti-shrimp allergen mAb was diluted at 1 μg/ml with coating solution and used to coat 96-well micro-plates at 100 ng/well. After blocking and washing, 100 μl of the Biotinylated-Ab2β-1D5 (40 ng/ml) was added to specifically test their ability to bind the anti-ZEN mAb.