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Bioceramics for Development of Bioartificial Liver
Published in Severian Dumitriu, Valentin Popa, Polymeric Biomaterials, 2020
Tomokazu Matsuura, Mamoru Aizawa
Porous cellulose beads are soft and prone to being squashed when used as a scaffold for RFB, which would make scaling-up of the bioreactor impossible. However, porous cellulose beads are suitable for use as the scaffold for RFB of 5 mL volume for experimental use. In particular, they are suitable for morphological observations due to their softness, thus they allow easy access to histological observation, immunostaining, and electron microscopic observation. We could culture the cells of Huh-7, a human hepatocellular carcinoma cell line, three-dimensionally for 2 months in RFB filled with porous cellulose beads, whose particle diameter was adjusted to approximately 1 mm (Figure 26.10).
Tunable nonenzymatic degradability of N-substituted polyaspartamide main chain by amine protonation and alkyl spacer length in side chains for enhanced messenger RNA transfection efficiency
Published in Science and Technology of Advanced Materials, 2019
Mitsuru Naito, Yuta Otsu, Rimpei Kamegawa, Kotaro Hayashi, Satoshi Uchida, Hyun Jin Kim, Kanjiro Miyata
β-Benzyl-l-aspartate N-carboxyanhydride (BLA-NCA) was purchased from Chuo Kaseihin Co. Inc. (Tokyo, Japan). n-Butylamine and 1,3-diaminopropane (DAP) were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). N-Methyl-2-pyrrolidone (NMP), benzene, N,N-dimethylformamide (DMF), dichloromethane (CH2Cl2), thiourea, lithium bromide (LiBr), dimethyl sulfoxide-d6 (containing 0.05 vol% tetramethylsilane), ethylenediamine (EDA), 1,4-diaminobutane (DAB), sodium chloride (NaCl), acetic acid, deuterium oxide, sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4, glycine, sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate 12-water, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, and Dulbecco’s phosphate-buffered saline (D-PBS) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was purchased from Dojindo Laboratories (Kumamoto, Japan). Trypsin-ethylenediamine tetraacetate and penicillin-streptomycin (P/S) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). EDA, DAP, DAB, NMP, and DMF were distilled with calcium hydride (CaH2) under reduced pressure. CH2Cl2 was distilled with CaH2 under normal pressure. A human hepatoma cell line (Huh-7) was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). Huh-7 cells were cultured in DMEM with 10% FBS (v/v) containing 1% P/S and incubated in 5% CO2 at 37 °C.