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New Insights into Feruloyl Esterase
Published in Jitendra Kumar Saini, Surender Singh, Lata Nain, Sustainable Microbial Technologies for Valorization of Agro-Industrial Wastes, 2023
Plate assay is an easy technique to screen a large number of microorganisms for different primary and secondary metabolites. Lai et al. (2012) incorporated 0.1% ethyl ferulate as a model substrate for feruloyl esterase enzyme into the de Man, Rogosa, and Sharpe (MRS) plate medium for the screening of a Lactobacillus strain. The appearance of the halo/clear zone around the inoculated colony is due to the hydrolysis of ethyl ferulate to show the presence of feruloyl esterase activity. Donaghy et al. (1998) also used de Man, Rogosa, and Sharpe (MRS) plate medium and screened 80 Bacillus strain and 50 gram bacterial strains such as Brevibacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus firmus, Bacillus licheniformis, Bacillus megaterium, Bacillus mycoides, Bacillus sphaericus, and Bacillus subtilis. Furthermore, gram-positive bacteria, including Enterococcus faecium, E. divas, Enterococcus faecalis, L. farciminis, L. delbrueckii, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus sakei, Lactococcus spp., and Leuconostoc spp., were screened on agar plate incorporated with ethyl ferulate. A clear zone around the colonies suggests the presence of feruloyl esterase activity.
Design, development and evaluation of a pilot-scale microwave based technology for sludge sanitization and drying
Published in Peter Matuku Mawioo, Novel Concepts, Systems and Technology for Sludge Management in Emergency and Slum Settings, 2020
Figure 7-11 shows the reduction profiles for the pathogens (selected indicator microorganisms) as function of the input MW energy for each type of sludge. The MW treatment was able to achieve complete destruction (below detection limit, i.e. <500 CFU/g TS or approximately 5.90 log removal value (LRV)) of pathogens of interest in the sludge at the conditions evaluated in this work. In all samples, the destruction of pathogens increased with increasing MW energy. For instance, in all samples when the sludge was exposed at 1.7 kWh, an over 3 LRV was achieved for the coliforms and enterococcus faecalis bacteria. However, when the exposure to MW energy was raised to 3.4 kWh, a reduction beyond the detection limit was achieved.
Textile Finishing
Published in Tom Cassidy, Parikshit Goswami, Textile and Clothing Design Technology, 2017
Andrew J Hebden, Parikshit Goswami
Modern health care provides a wealth of opportunities for both preventative methods to halt the spread of infection, be that antimicrobial textile surfaces, specialized wound dressings, or textile-based implantable material such as sutures, in which case biocompatibility is important. Healthcare-associated infections are a growing issue within modern medicine, and textiles can offer novel solutions to maintain cleanliness. Surface attachment and subsequent biofilm formation are a key survival mechanism for bacteria such as Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. The usage of nonwoven textile wipes offers a cheap and effective mechanism to remove bacterium from such surfaces. Plasma treatment of the nonwoven fibers with hexafluroethane (C2F6) leads to a reduction in the surface energy of the fibers, which increases removal efficiency of E. coli.19
Antimicrobial effectiveness of root canal sealers against Enterococcus faecalis
Published in Biomaterial Investigations in Dentistry, 2022
Paola Castillo-Villagomez, Elizabeth Madla-Cruz, Fanny Lopez-Martinez, Idalia Rodriguez-Delgado, Jorge Jaime Flores-Treviño, Guadalupe Ismael Malagon-Santiago, Myriam Angelica de La Garza-Ramos
Enterococcus faecalis is a common microorganism found in persistent asymptomatic endodontic infections. Its prevalence in these infections is 24% to 77% [6]. Root canal treatment failure is usually caused by a refractory intracanal infection or microorganisms invading the canal from the apical portion of the tooth [6]. E. faecalis within the root canals is one of the organisms that may cause post-treatment failures [7]. This bacterial penetration is largely attributed to the limitations of current disinfection protocols to combat intracanal E. faecalis infection [8].