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Bioinformatics
Published in Hajiya Mairo Inuwa, Ifeoma Maureen Ezeonu, Charles Oluwaseun Adetunji, Emmanuel Olufemi Ekundayo, Abubakar Gidado, Abdulrazak B. Ibrahim, Benjamin Ewa Ubi, Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics, 2022
O. M. Oyawoye, H. A. Olorunsola, A. O. Oluduro, O. O. Bamigboye, E. K. Oladipo, T. M. Olotu, I. J. Adeosun, M. O. Kaka, O. O. Obembe
The primary goal of waste removal using bioinformatics approach is to classify and analyze the DNA sequencing of microbes to use them for sewage cleaning, hazardous waste removal, oil spill clean-up, and other applications (Edu, 2020). Bacteria and other species assist in the clean-up of debris in bioinformatics. Deinococcus radiodurans, for example, can isolate degraded DNA and small fragments from chromosomes and repair them (Dahiya, 2017). By isolating damaged segments clustered region, this bacterium may fix affected DNA and small chromosome fragments. This is because it has many versions of the genome. To clear up the area, D. radiodurans has had genes from other bacteria introduced into it. It was used to break down organic compounds, solvents, and heavy metals in hazardous waste facilities (Dahiya, 2017).
Hybrid Gold Nanoparticles
Published in Vladimir Torchilin, Handbook of Materials for Nanomedicine, 2020
Alireza Gharatape, Roya Salehi
Additionally, microorganisms are another choice to produce GNPs based on green approaches. Two studies are described in detail in the following and others are listed in Table 1.1. Deinococcus radiodurans is a bacterium that can survive in high-stress conditions such as radiation and oxidants [49]. Jiulong and colleagues studied D. radiodurans and synthesized GNPs based on the microorganism approach. Then, the antibacterial activity of obtained nanoparticles was evaluated on two stains of Staphylococcus aureus and Escherichia coli. The average size and polydispersity index of particles measured by DLS were 43.75 nm and 0.23, respectively. Commonly, the antibacterial activity of synthesized GNPs shows that the Gram-negative and rod-shape E. coli are more sensitive than Gram-positive and spherical-shape S. aureus. Also, the results showed the damaging effects of GNPs were on the cytoplasmic membrane of both pathogenic bacteria. It seems that the authors used a large number of nanoparticles to confirm an antibacterial activity of nanoparticles. It was more suitable that they compare results of both synthetic methods (chemical and green) in the evaluation of antibacterial activity. Certainly, this study should be developed to provide a more conclusive result [49].
Heavy Metals, Hydrocarbons, Radioactive Materials, Xenobiotics, Pesticides, Hazardous Chemicals, Explosives, Pharmaceutical Waste and Dyes Bioremediation
Published in Vivek Kumar, Rhizomicrobiome Dynamics in Bioremediation, 2021
Elżbieta Wołejko, Agata Jabłońska-Trypuć, Andrzej Butarewicz, Urszula Wydro
Radiation resistance of Deinococcus radiodurans does not mean full tolerance to radioisotopes (Egas et al. 2014). The main reason for Deinococcus radiodurans high resistance to radiation seems to be not so much the prevention of DNA degradation, but rather the rapid and effective ability to remove serious damage in the genome. Bacteria that also show high resistance to radiation include Methylobacterium radiotolerans, Rubrobacter radiotolerans and cyanobacteria of the genus Chroococcidiopsis (Billi et al. 2000, Egas et al. 2014).
Phytoremediation of radioactive elements, possibilities and challenges: special focus on agricultural aspects
Published in International Journal of Phytoremediation, 2023
Gursharan Singh, Surabhi Bhadange, Fnu Bhawna, Pratiksha Shewale, Rahul Dahiya, Ashish Aggarwal, Fnu Manju, Shailendra Kumar Arya
It is the technique of bioremediation in which natural or genetically engineered bacterial strain is being used to treat the contaminated area. This method is often used when the species for bioremediation does not exist in the treatment place. In Genetic engineering, Deinococcus radiodurans has much efficient role in bioremediation of radioactive elements (Shukla et al. 2017).
Whole-cell bioreporters for evaluating petroleum hydrocarbon contamination
Published in Critical Reviews in Environmental Science and Technology, 2021
Bo Jiang, Yizhi Song, Zengjun Liu, Wei E. Huang, Guanghe Li, Songqiang Deng, Yi Xing, Dayi Zhang
Theoretically, either bacteria, yeast or mammalian cells can be used as the host for WCB construction. Among them, bacterial strains are the most popular ones owing to their easiness in rapid cultivation. The commonly used host bacteria of WCBs are Escherichia coli (Baumann & van der Meer, 2007; Lewis et al., 2008; Truffer et al., 2014) and Salmonella typhimurium (Aßmann, Emmrich, Kampf, & Kaiser, 1997; Petrilli & De Flora, 1977; van der Lelie, Regniers, Borremans, Provoost, & Verschaeve, 1997). With fully sequenced whole genome and mature genetic manipulation protocols (Blattner et al., 1997), they are ideal candidates for constructing WCBs and optimizing their performance. Soil microbes such as Acinetobacter baylyi ADP1 (Song et al., 2009) and Pseudomonas putida (He et al., 2018; Kuncova, Pazlarova, Hlavata, Ripp, & Sayler, 2011; Li, Peng, Su, & Tao, 2014; Shingler & Moore, 1994; Trogl et al., 2012) are also used as hosts for WCBs. As they are indigenous microbes in environment, their response to environmental contaminants is more representative for evaluating environmental risks comparing to model E. coli strains for bioreporter construction (DH5α or JM109), which are enteric microorganisms and can only achieve optimal response at 37 °C and in rich medium. Besides, other strains with the ability to tolerate high levels of environmental contaminants or stresses are also feasible as hosts for WCBs. For example, Gao et al. designed a genotoxicity WCB using Deinococcus radiodurans with recA promoter (Gao et al., 2008), demonstrating its adaptation to high intensity of radiation and application for genotoxicity assessment in radioactive cases. Novosphingobium sp. HR1a can grow with diverse PAHs as the sole carbon source and then used as WCB responsive in the presence of PAHs and their intermediates, constructed using lacZ gene fused with the PAHs-degrading pathway promoter (Segura, Hernandez-Sanchez, Marques, & Molina, 2017). Appropriate selection of host strains can significantly broaden the applicable ranges of WCBs in realistic environmental conditions.