China
Africa
Explore chapters and articles related to this topic
Genes and genomics
Published in Firdos Alam Khan, Biotechnology Fundamentals, 2018
Ligation-mediated PCR uses small DNA linkers ligated to the DNA of interest and multiple primers annealing to the DNA linkers. This method is deployed for DNA sequencing, genome walking, and DNA footprinting. A related technique is the AFLP, which generates diagnostic fragments of a genome.
DNA-interaction studies of a copper(II) complex containing ceftobiprole drug using molecular modeling and multispectroscopic methods
Published in Journal of Coordination Chemistry, 2018
Nahid Shahabadi, Soraya Moradi Fili
Metal complexes have been widely applied in medicine for centuries, although their molecular mechanism has not yet been fully understood [6, 7]. Metal ions present in complexes not only accelerate drug action by forming coordination complexes with them, but also enhance the effectiveness of the organic ligands [8, 9]. The medicinal properties of metal complexes depend on the nature of the metal ions and the ligands [10]. Interest in preparation of new metal complexes has forced the study of the interaction of metal complexes with DNA for their potential applications in medicine [11]. Copper is a biologically relevant element and many enzymes that depend on copper for their activity have been identified. Because of its biological relevance, a large number of copper(II) complexes have been synthesized and explored for their biological activities [12]. Artificial nucleases will provide important new tools for DNA manipulation to molecular biologists. For example, [bis(phenanthroline)copper(II)] complex is used in DNA-footprinting experiments, which are important for the detailed study of DNA-protein interactions. The role of ternary copper(II) complexes in biological systems is well known. Recently, there have been several reports of copper(II) complexes showing efficient chemical nuclease activity. Recent reports have shown that amino acid/peptide-based copper(II) complexes show efficient DNA-cleavage activity by oxidative and hydrolytic pathways [13].