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Extremophiles Life of Microorganisms in Extreme Environments
Published in Pratibha Dheeran, Sachin Kumar, Extremophiles, 2022
Rahul Kumar, Ramchander Merugu, Swati Mohapatra, Sneha Sharma, Hemlata
Enzymes obtained from extreme heat-loving microorganisms are of paramount importance as they act as biocatalysts for various industrial applications. Such enzymes can work effectively even at extreme high temperatures (Lopez et al. 2013). The bacterial species such as Bacillus subtilis have antidiarrhoeal properties. This species is also used to produce fermented soybeans, yogurt, ice cream, milk and cheese. The bacterial species Streptococcus thermophilus has a property to boost the immunity of a human body and can also be used in the fermentation of cheese. Brevibacillus borstelensis can be used for the degradation of polyethylene and long-chain hydrocarbons (Hadad et al. 2005, Khalil et al. 2018). Geobacillus stearothermophilus showed significant esterase producing ability, hence it can be used for the production of thermostable esterase (Ghati et al. 2013, Pathak and Rathod 2014). Other applications of hyperthermophiles are: Bioethanol production: Lignocellulose is a non-edible plant which is mostly used in the production of bioenergy or biofuel (Hood 2016). Cellulose is the most abundant and renewable non fossil carbon source. Hyperthermophilic cellulose enzyme produced by Pyrococcus horikoshii is used for cellulose degradation (Wang et al. 2011). Hyperthermophiles are used in cellulytic reaction for sustainable bioethanol production.Starch processing: Starch bioprocessing usually involves high temperature dependent two steps (Pervez et al. 2014): LiquefactionSaccharification
Effect of nutritional parameters on microbial production of L-Asparaginase by novel Brevibacillus borstelensis ML12
Published in Preparative Biochemistry & Biotechnology, 2023
Rupkatha Mukherjee, Debabrata Bera
50 mL of nutrient broth was inoculated with 2%, 24-hour-old inoculum of Brevibacillus borstelensis ML12 culture in 250 mL Erlenmeyer flask at 37 °C and 120 rpm and O.D. values were recorded from 0 minute till 120 minutes. Pre-weighed aluminum cups were used to collect the cell mass and the difference in weight was noticed. Cell mass was validated with cell number.