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Introduction to Biological Light Microscopy
Published in Jay L. Nadeau, Introduction to Experimental Biophysics, 2017
Jay L. Nadeau, Michael W. Davidson
The most popular traditional probes for endoplasmic reticulum fluorescence analysis are the carbocyanine and xanthene dyes, DiOC(6) and several rhodamine derivatives, respectively. These dyes must be used with caution, however, because they can also accumulate in the mitochondria, Golgi apparatus, and other intracellular lipophilic regions. Newer, more photostable probes have been developed for selective staining of the endoplasmic reticulum by several manufacturers. In particular, oxazole members of the Dapoxyl family produced by Molecular Probes are excellent agents for selective labeling of the endoplasmic reticulum in living cells, either alone or in combination with other dyes. These probes are retained after fixation with formaldehyde, but can be lost with permeabilizing detergents. Another useful probe is Brefeldin A, a stereochemically complex fungal metabolite that serves as an inhibitor of protein trafficking out of the endoplasmic reticulum.
Transferrin/folate dual-targeting Pluronic F127/poly(lactic acid) polymersomes for effective anticancer drug delivery
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Qing Xiao Wang, Xiang Chen, Zi Ling Li, Yan Chun Gong, Xiang Yuan Xiong
Besides, Brefeldin A could block the transport of the endoplasmic reticulum to the Golgi apparatus. Compared with the control group, the cell uptake rate of coumarin 6-loaded Ps decreased significantly. Consequently, the endocytosis of Ps was related to the endoplasmic reticulum to the Golgi apparatus [55]. Monensin could block the transport of endosomes to lysosomes [54]. The uptake amount of coumarin 6-loaded Ps dropped, which indicated that their endocytosis was related to endosomes and lysosomes [56, 57].