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Biological Analysis of Fungi and Associated Molds
Published in Christopher S. Cox, Christopher M. Wathes, Bioaerosols Handbook, 2020
Histoplasmosis, caused by the dimorphic fungus Histoplasma capsulatum, is sometimes fatal to man and animals, and is apparently acquired by the airborne route. A benign form of this disease also occurs. It has been isolated from air inside and outside chicken houses by means of a novel and highly selective technique in which particles were washed from the air by “venturi scrubbers” (intended for use industrially for cleaning air), the solid material collected by filtration, resuspended in saline with bactericidal antibiotics and injected into mice intraperitoneally. Four weeks after inoculation, the mice were sacrificed and liver, spleen and adrenals, after sterile maceration, were plated out onto antibiotic containing culture media suitable for H. capsulatum on which colonies grew.97 Microscopic examinations of clinical material are unrewarding. A recommended culture medium is brain heart infusion-cycloheximide-chloramphenicol(BHI-CC) and BHI-CC fortified with 6% blood.96 Skin tests with histoplasmin may be inconclusive.
Overproduction of thermoalkalophilic lipase secreted by Bacillus atrophaeus FSHM2 using UV-induced mutagenesis and statistical optimization of medium components
Published in Preparative Biochemistry and Biotechnology, 2019
Atefeh Ameri, Mojtaba Shakibaie, Mozhde Soleimani-Kermani, Mohammad Ali Faramarzi, Hamid Forootanfar
ρ-nitrophenyl palmitate (ρ-NPP) and ρ-nitrophenol (ρ-NP) were provided by Sigma-Aldrich (St. Louis, MO, USA). Peptone, Rhodamine B, nutrient broth (NB), nutrient agar, yeast extract, and brain heart infusion broth (BHIB), as well as glucose and sucrose, were bought from Merck Chemicals (Darmstadt, Germany).