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Omics and female reproduction
Published in Moshe Hod, Vincenzo Berghella, Mary E. D'Alton, Gian Carlo Di Renzo, Eduard Gratacós, Vassilios Fanos, New Technologies and Perinatal Medicine, 2019
Morphological assessment of the zona pellucida (ZP) is the gold standard method in oocyte selection in most ART clinics, but this approach is limited by precision boundaries. Studies have shown that differentially expressed candidate genes were respectively overexpressed and underexpressed in cumulus/granulosa cells from oocytes that led to a successful pregnancy, versus oocytes that did not (20,21). ZP properties’ variation is associated with differences in cumulus/granulosa cell gene expression, but ZP morphology is associated with a transcriptomic gene pattern that is not directly related to known gene biomarkers of oocyte development. Further studies using larger lists of candidate markers are required to identify suitable genes highly correlated with ZP morphological criteria, in order to reinforce the accuracy of oocyte selection and potentially increase ART success rates (19).
Oocyte retrieval and selection
Published in David K. Gardner, Ariel Weissman, Colin M. Howles, Zeev Shoham, Textbook of Assisted Reproductive Techniques, 2017
Laura F. Rienzi, Filippo M. Ubaldi
On the basis of the above-mentioned potentialities of oocyte selection tools, future perspectives for the betterment of assisted reproductive techniques reside in creating a direct connection between IVF laboratories and research units. The so-called “omics technologies” (transcriptomic, proteomic, and metabolomic) is gaining increased interest from the scientific community. The results obtained so far are intriguing and encouraging, but it would be wise to raise some concerns. In fact, the high costs, difficulty of the techniques, and time required for testing are currently limiting their routine applicability. Moreover, it is of the utmost importance to provide definitive evidence of the bio-safety of these techniques, and more studies are required in order to determine their predictive power (115, 117).
Follicular fat-soluble vitamins as markers of oocyte competency
Published in Systems Biology in Reproductive Medicine, 2020
Patrycja Skowrońska, Michał Kunicki, Ewa Pastuszek, Lucyna Konieczna, Tomasz Bączek, Krzysztof Łukaszuk
The vast majority of data about molecules in FF comes from studies on concentrations of AMH and other hormones such as progesterone (Kobayashi et al. 1991; Fanchin et al. 2007; Revelli et al. 2009; Kedem-Dickman et al. 2012; Lin et al. 2013) and vitamin D (Anifandis et al. 2010; Ciepiela et al. 2018; Abedi et al. 2019; Zhao et al. 2019). Effects of other vitamins are less known. Their evaluation may add new knowledge to currently used methods of oocyte selection and prediction of embryo development such as morphological assessment, time-lapse imaging or preimplantation genetic testing (Revelli et al. 2009).
Vitamin D and anti-Müllerian hormone concentration in human follicular fluid individually aspirated from all patient follicles
Published in Gynecological Endocrinology, 2022
Patrycja Skowrońska, Michał Kunicki, Ewa Pastuszek, Lucyna Konieczna, Tomasz Bączek, Błażej Męczekalski, Roman Smolarczyk, Krzysztof Łukaszuk
Follicular fluid (FF) is the microenvironment for the oocyte’s development [1]. Evaluation of concentration of the anti-Müllerian hormone (AMH) and vitamin D found in FF may add new information for use in oocyte selection. This may be particularly important when the number of oocytes to be fertilized has to be limited, e.g. for legal reasons.