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Ovum Pick-Up (OPU) in Cattle: An Update
Published in Juan Carlos Gardón, Katy Satué, Biotechnologies Applied to Animal Reproduction, 2020
According to the International Embryo Transfer Society (IETS) statistics, the number of embryos produced in vitro and transferred into recipients has increased in the last years. This outstanding growth of OPU/IVEP seen in the last years is a consequence of a number of factors, such as the significant improvement of in vitro culture procedures and the successful use of sex-sorted semen in the IVEP programs enabling the manipulation of the proportion of male and female embryos produced (Blondin, 2015).
Embryo Transfer
Published in Arianna D'Angelo, Nazar N. Amso, Ultrasound in Assisted Reproduction and Early Pregnancy, 2020
It is important to explain the process of embryo transfer in advance. Patients need to be reassured that even though it is one of the most important steps of their journey, it is a quite short and simple procedure with little or no discomfort. A positive attitude could help alleviate undue anxiety that has been shown to be associated with a higher frequency of uterine contractions [8]. Advance knowledge of additional risk factors such as difficulties during intimate examinations would ensure that necessary steps are undertaken to make the process less stressful and more straightforward.
In Vitro Fertilization and Embryo Transfer
Published in Asim Kurjak, Ultrasound and Infertility, 2020
To ameliorate the quality of prepared material, unactivated homologised or fetal serum is added into the medium. For every patient, three different media are to be prepared: Medium for insemination. Medium is used for oocyte maturation and sperma preparation. It contains between 7.5 and 10% of unactivated serum.Medium for embryonal growth. It is used for the development and growth of the embryo following insemination. In this medium, between 15 and 20% of serum is added.Medium for embryo transfer is used mainly for transfer of already developed embryos into the uterus. It contains 75 to 90% of serum.
Pregnancy in women with congenital heart disease: a focus on management and preventing the risk of complications
Published in Expert Review of Cardiovascular Therapy, 2023
Gurleen Wander, Johanna A. van der Zande, Roshni R Patel, Mark R Johnson, Jolien Roos-Hesselink
If there is a history of subfertility and the patient is considering further investigation of the underlying cause or assisted reproduction techniques (ART), it is important to discuss the investigations and the risks that they pose. For example, diagnostic laparoscopy and the associated pneumoperitoneum or hysteroscopy (needing fluid distension of uterine cavity) can cause life threatening compromise to a woman with a univentricular circulation or pulmonary hypertension. It is therefore important to liaise with the gynecologist who will be undertaking this care and give clear guidance regarding the cardiac tolerance of the patient. Ovarian hyperstimulation syndrome associated with ART, is pro thrombotic and associated with marked fluid shifts that could cause further cardiac complications. A single embryo transfer should be recommended after ART, especially in women with cardiac disease, as a multiple pregnancy is associated with greater cardiovascular changes and increased risks of fetal and maternal morbidity.
The effect of endometrial scratching on reproductive outcomes in infertile women undergoing IVF treatment cycles
Published in Journal of Obstetrics and Gynaecology, 2022
Narjes Noori, Faezeh Ghaemdoust, Marzieh Ghasemi, Mahsa Liavaly, Narjes Keikha, Javid Dehghan Haghighi
Between Day 18 and Day 24 after the randomisation process, for patients in the intervention group, ES was performed by a Pipelle curette in the cycle preceding the frozen embryo transfer. In contrast, this procedure was not performed for the control group The luteal phase was selected for ES regarding similar study methods (Yeung et al.2014, Frantz et al.2019). The same infertility expert performed all endometrial scratches. On the second or third day of the next menstrual cycle, ultrasonic evaluation was performed, and in case of ovarian suppression and absence of dominant follicles, Oestradiol Valerate 6 mg/d was administered for ten days. Subsequently, ultrasound evaluation of the endometrial thickness (ET) was conducted, and the oestradiol dosage was adjusted (if the ET was not proper for the procedure (ET less than 8 mm), dose-adjustment was done (8 mg), and another ultrasonography was performed 3−5 d later to evaluate whether the ET is ready). Once the ET reached 8 mm or more, micronised progesterone (endometrin-vaginal insert 100 mg Ferring) administration was started three times a day. Two or three eight-cell embryos of grade A or B were transferred after completing the three days of progesterone administration (on the fourth day). Embryo transfer was done with ultrasound guidance and by a cook catheter.
Mid-luteal uterine artery Doppler indices in the prediction of pregnancy outcome in nulliparous women undergoing assisted reproduction
Published in Human Fertility, 2022
David A. Crosby, Louise E. Glover, Paul Downey, Eoghan E. Mooney, Fionnuala M. McAuliffe, Cliona O'Farrelly, Donal J. Brennan, Mary Wingfield
A total of 98 women were eligible to participate in the study. Of this cohort, 55 (56.1%) underwent a transvaginal ultrasound in the cycle preceding the planned ART cycle. Of these, five (9.1%) women did not undergo an embryo transfer in the subsequent cycle and were excluded. There was a 42.0% clinical pregnancy rate per embryo transfer (‘pregnant’, n = 21/50) and 58.0% (n = 29/50) had an unsuccessful outcome (‘not pregnant’). The patient characteristics of the two groups are outlined in Table 1. All women were nulliparous. There were no significant differences in mean age, body mass index (BMI), duration of infertility, cycle length or serum Anti-Müllerian Hormone (AMH) between the two groups. Furthermore, there were no differences in fertility aetiology or number of previous ART cycles prior to study cycle. ART cycle characteristics are outlined in Table 2. There were no differences between the two groups.