Sulfanilic acid

Methods to Study Endothelium-Dependent Responses

Published in Thomas F. Lüscher, Paul M. Vanhoutte, The Endothelium: Modulator of Cardiovascular Function, 2020

Thomas F. Lüscher, Paul M. Vanhoutte

In an alternative method, the assay is based on the diazotization of sulfanilic acid by nitric oxide at acidic pH and subsequent coupling with N-( 1 -naphthyl)-ethylenediamine which yields a colored product that can be measured spectrophotometrically.69,545,551

Methods of Protein Iodination

Published in Erwin Regoeczi, Iodine-Labeled Plasma Proteins, 2019

Erwin Regoeczi

Primary aryl amines can be converted into their reactive diazonium salts by nitrous acid (HNO2) in the presence of dilute HC1. This may be illustrated for sulfanilic acid as follows:

Published in Ronald M. Atlas, James W. Snyder, Handbook Of Media for Clinical Microbiology, 2006

Ronald M. Atlas, James W. Snyder

Use: For the differentiation of aerobic and facultative Gram-negative microorganisms based on their ability to reduce nitrate. Test for nitrates with sulfanilic acid and α-naphthylamine reagents. Bacteria that reduce nitrate to nitrite turn the reagents red or pink.

Protective role of resveratrol and apigenin against toxic effects of bisphenol a in rat salivary gland

Published in Drug and Chemical Toxicology, 2023

Yaser Said Çetin, Fikret Altındağ, Mehmet Berköz

Malondialdehyde (MDA) level was measured according to the method reported by Yagi. MDA reacts with thiobarbituric acid (TBA) and yields a spectroscopically readable final product at 532 nm. MDA levels were expressed as nmol/mg protein using the extinction coefficient value of 1.56 × 105 M−1.cm−1 (Yagi 1998). Protein carbonyl level was estimated using the method described by Levine et al. (1990). The homogenates were incubated with 2,4-dinitrophenylhydrazine (DNPH) in 6 M guanidine hydrochloride. As DNPH binds selectively to the protein carbonyl groups, the absorbance at 370 nm changes. The level of protein carbonyls was expressed as nmol/mg of protein. Nitric oxide concentration in the salivary gland tissue was measured using the Griess reagent. The principle of the assay is based on the conversion of sulfanilic acid to a diazonium salt by reaction with nitrite in acid solution. The diazonium salt is then coupled to N- (1-naphthyl) ethylenediamine, forming an azo dye that can be quantified through colorimetry at 540 nm in microplate reader and calculated against a sodium nitrite standard curve (Miranda et al.2001).

Prophylactic effect of Biochanin A in lipopolysaccharide-stimulated BV2 microglial cells

Published in Immunopharmacology and Immunotoxicology, 2020

Mehmet Berköz, Mirosław Krośniak, Ferbal Özkan-Yılmaz, Arzu Özlüer-Hunt

The principle of the assay is based on the conversion of sulfanilic acid to a diazonium salt by reaction with nitrite in acid solution. The diazonium salt is then coupled to N-(1-naphthyl) ethylenediamine, forming an azo dye that can be quantified through colorimetry. BV2 cells were seeded out in a 96-well plate at a density of 2 × 105 cells/mL for 48 h. Cells were then stimulated with LPS (100 ng/mL) [27] in the absence or presence of nontoxic Biochanin A concentrations (2.5, 5 and 10 μM) and for 24 h. The untreated cells were incubated with only DMEM containing 0.5% DMSO. Cell supernatants were collected and centrifuged for 5 min at 1500 rpm. Nitrite concentration in the culture medium was measured using Griess assay kit (Promega). Culture supernatants (50 μL) were added to a 96-well microtest plate. Thereafter, 50 μL of sulfanilamide was added to the plate and incubated in the dark for 10 min. Then, 50 μL of N-(1-naphthyl) ethylenediamine (NED) was added and incubated for 10 min. Absorbance was read at 540 nm in a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) and calculated against a sodium nitrite standard curve [25].

Oxidative stress, NOx/l-arginine ratio and glutathione/glutathione S-transferase ratio as predictors of ‘sterile inflammation’ in patients with alcoholic cirrhosis and hepatorenal syndrome type II

Published in Renal Failure, 2018

Vanja P. Nickovic, Dijana Miric, Bojana Kisic, Hristina Kocic, Marko Stojanovic, Salvatore Buttice, Gordana Kocic

General biochemical parameters were determined by biochemical IFCC methods (kinetic spectrophotometric methods on multi-channeled biochemical OLYMPUS-AU 680 analyzer). Determining MDA plasma concentration was obtained by Ledwozyw et al. [23] method. This method is a colorimetric determination of MDA using thiobarbituric acid. Determining GSH plasma concentration was obtained using the Sedlak and Lindsay method [24], a colorimetric determination of GSH based on Elman reagent reaction (2-nitrobenzene acid) and SH group of GSH. Additionally, determining GST plasma activities was done with the Habig method [25] that is based on the GST capability to catalyze conjugation reaction of GSH with substrate 1-chlor 2.4-dinitrobenzene (CDNB). Determination of NO2 + NO3 concentration was performed using the Navaro-Gonzalvez et al. [26] semiautomated method, based on diazotization of sulfanilic acid.