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Order Tymovirales
Published in Paul Pumpens, Peter Pushko, Philippe Le Mercier, Virus-Like Particles, 2022
Paul Pumpens, Peter Pushko, Philippe Le Mercier
The fluorescent dye OregonGreen 488 was conjugated to the PVX particles using both Lys-N-hydroxysuccinimide and Cys-maleimide chemistry, with the former achieving the best performance resulting in the modification of up to 15% of the coats (Le et al. 2017a). This may reflect the low accessibility of the Cys residue, which was thought to be located within a surface groove (Röder et al. 2019).
Enzymatic Syntheses of Biologically Active Peptides
Published in Willi Kullmann, Enzymatic Peptide Synthesis, 1987
In addition to the above-mentioned MSH fragments, another tetrapeptide adjacent to the N-terminus of the “active core” of dogfish β-MSH49 was synthesized. The synthetic pathway leading to the tetrapeptide Boc-Tyr-Lys-Phe-Gly-N2H2Ph (Figure 11) was suggested by the presence of two aromatic residues resulting in putative chymotrypsin-sensitive bonds in the primary structure. This feature was exploited for the chymotryptic synthesis of the dipeptides Boc-Tyr-Lys-N2H2Ph (56%) and Boc-Phe-Gly-N2H2Ph (68%) (Figure 11). After rearranging Boc-Tyr-Lys-N2H2Ph to the corresponding dipeptide methyl ester, the latter was reacted with H-Phe-Gly-N2H2Ph in the presence of trypsin to give Boc-Tyr-Lys-Phe-Gly-N2H2Ph in 60% yield.
Proteomic exploration of cystathionine β-synthase deficiency: implications for the clinic
Published in Expert Review of Proteomics, 2020
CBS deficiency essentially eliminates the transsulfuration pathway (ii) and increases the flow of Hcy through the re-methylation (i) and the Hcy-thiolactone (iii) pathways (Figure 1). As a result, Met, Hcy, and related metabolites such as Hcy-thiolactone, N-Hcy-Lys, N-Hcy-protein, and S-Hcy-protein are severely elevated in CBS deficiency both in humans and mice (Table 1).