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Orders Norzivirales and Timlovirales
Published in Paul Pumpens, Peter Pushko, Philippe Le Mercier, Virus-Like Particles, 2022
Paul Pumpens, Peter Pushko, Philippe Le Mercier
Recently, Bhushan et al. (2018) proposed S-allylhomocysteine as an unnatural genetically encodable methionine analog for the Qβ VLPs. This analog is processed by translational cellular machinery and is also a privileged olefin cross-metathesis reaction tag in proteins. It was used for efficient Met-codon reassignment in a Met-auxotrophic strain of E. coli.
Chimeric VLPs
Published in Paul Pumpens, Single-Stranded RNA Phages, 2020
Recently, Bhushan et al. (2018) proposed S-allylhomocysteine as an unnatural genetically encodable methionine analog for the Qβ VLPs. This analog is processed by translational cellular machinery and is also a privileged olefin cross-metathesis reaction tag in proteins. It was used for efficient Met-codon reassignment in a Met-auxotrophic strain of E. coli.
An efficient system for bioconjugation based on a widely applicable engineered O-glycosylation tag
Published in mAbs, 2021
Thomas V. Murray, Kasia Kozakowska-McDonnell, Adam Tibbles, Annabel Taylor, Daniel Higazi, Emmanuel Rossy, Alessandra Rossi, Sivaneswary Genapathy, Giulia Tamburrino, Nicola Rath, Natalie Tigue, Vivian Lindo, Tristan Vaughan, Monika A. Papworth
Numerous strategies have been developed that co-opt the endogenous biosynthetic machinery to generate biomolecules containing bio-orthogonal functional groups that can be used for site-specific bioconjugation.5 Such approaches exploit the endogenous pathways at three different levels: 1) transcriptionally by the expansion of the genetic code and codon reassignment;6 2) translationally by the evolution of bio-orthogonal aminoacyl-tRNA synthetase/tRNA pairs,7 which facilitate the incorporation of unnatural amino acids; and 3) post-translationally, by modifying glycosylation,8 phosphorylation,9 and lipidation10 patterns, among others. Metabolic glyco-engineering (MGE) approaches (reviewed by Agatemor et al.8) that manipulate cellular metabolism to generate functionalized glycoproteins have become an attractive method for the generation of bioconjugates because they bypass technical challenges of other techniques, such as the requirement for the transfection of multicomponent codon reassignment machinery for unnatural amino acid incorporation or complex post-production steps required by in vitro enzymatic post-translational approaches.