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Cholesterol in the Walls of Odontogenic Cysts: A Histochemical and Ultrastructural Study
Published in Roger M. Browne, Investigative Pathology of the Odontogenic Cysts, 2019
J. R. Garrett, K. M. S. Winstone
Oil Red O staining for neutral lipids has been shown to demonstrate triglycerides and cholesterol esters.18 It is also known to stain some free fatty acids. Its use led to intense globular staining of foamy cells, thereby showing them to advantage (Figure 2A) and so demonstrated that they had a far greater presence than could have been appreciated from paraffin sections. Cholesterol crystals that had remained in the sections and not fallen out were unstained by Oil Red O but they maintained their birefringent features. Use of crossed polars after Oil Red O staining showed that the foamy cells contained globular birefringent material (Figure 2B), which is attributable to crystalline cholesterol esters. Small intracellular needle-like crystals were also observed in some of the cells and are thought to be due to free cholesterol. Occasionally, red stained lipid droplets were identified in some giant cells associated with cholesterol clefts but most of the giant cells showed no lipid staining. There was a tendency for faint staining to occur within the fibrous tissue and this was thought to be due to triglycerides associated with collagen fibers.
Routine and Special Techniques in Toxicologic Pathology
Published in Pritam S. Sahota, James A. Popp, Jerry F. Hardisty, Chirukandath Gopinath, Page R. Bouchard, Toxicologic Pathology, 2018
Daniel J. Patrick, Matthew L. Renninger, Peter C. Mann
Oil Red O is used to demonstrate the presence of neutral lipids in tissue sections. This technique can only be used on unprocessed (unfixed frozen or formalin-fixed but unprocessed) tissue samples. If the tissue has undergone processing, the stain will not work, since the dehydration step will remove the lipid from the tissue. Oil Red O stains neutral lipids red.
How to master MCQs
Published in Chung Nen Chua, Li Wern Voon, Siddhartha Goel, Ophthalmology Fact Fixer, 2017
Sebaceous cell carcinoma of the upper lid is an aggressive tumour which can metastasise early. It typically presents as unilateral blepharitis or a recurrent chalazion. The upper lid is more commonly involved than the lower lid due to the larger number of meibomian glands in the upper lids. Risk factors include old age and previous radiation to the head. In a poorly differentiated tumour, it may be difficult to differentiate it from squamous cell carcinoma. However, the use of Sudan black or Oil red O can be useful as both will stain for the presence of fat in tumour cells seen in sebaceous cell carcinoma.
Inhibition of EZH2 mitigates peritoneal fibrosis and lipid precipitation in peritoneal mesothelial cells mediated by klotho
Published in Renal Failure, 2023
Qinglian Wang, Jingshu Sun, Rong Wang, Jing Sun
A 10 mM stock solution of EZH2 inhibitor (HY-13500, MedChemExpress Bio-Technology, Shanghai, China) was purchased and stored at −80 °C. The dilutions for the working solution did not exceed 0.1% DMSO in the medium. Dulbecco’s modified Eagle’s medium (DMEM F12/1:1), fetal bovine serum (FBS), and trypsin/EDTA were purchased from HyClone (Logan, UT, USA). Nile red (N8440) and Oil red O stains (G1262) were purchased from Solarbio Company. Klotho siRNA (sc-43883) was purchased from Santa Cruz Biotechnology. A tissue triglyceride assay kit (E1013) was purchased from Pplygen Company (Beijing, China). The primary antibodies were as follows: anti-EZH2 (ab191080, Abcam, Cambridge, MA, USA) for WB, as well as anti-E-cadherin (ab231303, Abcam, USA), anti-N-cadherin (ab76011, Abcam, USA), anti-vimentin (ab92547, Abcam, USA), anti-FN (ab2413, Abcam, USA), anti-klotho (ab181373, Abcam, USA) and anti-GAPDH antibodies. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse antibodies were provided by Beyotime Biotechnology (Shanghai, China). All other chemicals were of reagent grade and endotoxin free.
Aggravation of atherosclerosis by pulmonary exposure to indium oxide nanoparticles
Published in Nanotoxicology, 2020
Dong-Keun Lee, Hyung Seok Jang, Hyunji Chung, Soyeon Jeon, Jiyoung Jeong, Jae-Hoon Choi, Wan-Seob Cho
After the aortic sinus was cut into 4 µm thick slices by using a cryostat microtome (Leica Biosystems), the slices were dried for 1 h at room temperature and fixed in acetone for 5 min. The tissue sections were washed twice with PBS and fixed in 1 mL of fixative solution [2 parts of citrate working solution (2 mL citrate concentrate solution + 8 mL DW) and 3 parts of acetone] for 30 s, and the medium was discarded. Fixation was conducted in 1 mL of 70% ethyl alcohol for 30 s. After washing in DW, the sections were stained in 1 mL of staining solution [6 parts of oil red O stock solution (300 mg oil red O in 100 mL of 99% isopropanol) and 4 parts of DW] for 6–15 min, thoroughly washed using in DW, counterstained with 1 mL hematoxylin for 5 min, rinsed with DW, and mounted with glycerol. The sections were observed by using an Olympus BX54 microscope and the positive area was digitized and computed by using the DP74 built-in software (Olympus).
Esculin protects against methionine choline-deficient diet-induced non-alcoholic steatohepatitis by regulating the Sirt1/NF-
Published in Pharmaceutical Biology, 2021
Xi-Ding Yang, Zhuo Chen, Ling Ye, Jing Chen, Yong-Yu Yang
Esculin (purity ≥98%, lot # D1812002, purchased year: 2019) and oleic acid (lot # I1608084) were purchased from Aladding Company Ltd. (Shanghai, China). Oil Red O was (purity ≥75%, lot # 303C051, purchased year: 2018) purchased from Solarbio Science and Technology Company (Beijing, China). Lipofectamine 3000 Transfection Reagent was purchased from Invitrogen (Carlsbad, CA, USA). The anti-NF-κB p65 acetyl antibody was obtained from Abcam (Cambridge, MA, USA). The anti-fibronectin, anti-collagen 4A1, and anti-β-actin antibodies, and the TNF-α, IL-6, and IL-1β ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China). Triglyceride (TG), total cholesterol (TC), and free fatty acid (FFA) detection kits were procured from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Anti-Sirt1 antibody, anti-NF-κB p65 antibody, the bicinchoninic acid (BCA) protein assay kit, radioimmunoprecipitation assay (RIPA), phenylmethylsulfonyl fluoride (PMSF), NADPH/NADP+ ratio, and the BeyoECL plus kit were purchased from Beyotime Biotechnology (Shanghai China). TRIzol, SYBR® Premix Ex Taq™, and the PrimeScript reverse transcription reagent kit were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Methionine-choline deficient (MCD) diet and methionine and choline supplemented (MCS) diet (control diet) were purchased from Shulaibao Biotechnology Co., Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) and foetal bovine serum (FBS) were obtained from HyClone (Logan, UT, USA). FFA-free bovine serum albumin (BSA, lot # D00162664) and palmitic acid (lot # SLBG3957V) were procured from Sigma-Aldrich (St Louis, MO, USA).