China
Africa
Explore chapters and articles related to this topic
Advanced Glycation End Products—A Special Hazard in Diabetes
Published in Robert Fried, Richard M. Carlton, Type 2 Diabetes, 2018
Robert Fried, Richard M. Carlton
A study published in the American Journal of Kidney Diseases was conducted on long-term dialysis patients to determine the effects of dietary AGEs on renal function. Dietary AGE intake was estimated by means of dietary records and questionnaires, and sera were obtained for measurement of two well-characterized AGEs, CML and MG (methylglyoxal) derivatives. CML is N(6)-carboxymethyllysine, also known as N(ε)-(carboxymethyl)lysine, an AGE. It has been the most commonly used marker for AGEs in food analysis.
Cancer
Published in Stephen P. Coburn, The Chemistry and Metabolism of 4′-Deoxypyridoxine, 2018
Mihich and Nichol325 reported that intraperitoneal administration of deoxypyridoxine (50 to 100 mg/kg/day) decreased the growth of solid sarcoma 180 tumors in mice receiving either normal or B6-deficient diets. Mortality was also increased. Both effects were enhanced when methylglyoxal-bis (IM-methylthiosemicarbazone) was added to the treatment.
AGE-RAGE Axis in the Aging and Diabetic Heart
Published in Sara C. Zapico, Mechanisms Linking Aging, Diseases and Biological Age Estimation, 2017
Karen M. O’Shea, Ann Marie Schmidt, Ravichandran Ramasamy
Methylglyoxal, in particular, is one the most abundant precursors for AGEs and has been implicated in mediating the complications of diabetes (Shamsi et al. 1998). Diabetic patients with optimal glycemic control experience the development of complications due to elevated methylglyoxal (Turk 2010). Methylglyoxal is a 2-oxoaldehyde that can be derived from various metabolic pathways involving glycolytic intermediates, polyol pathway intermediates and ketone metabolism (Thornalley 1993). The formation of methylglyoxal from ketone metabolism may account for the especially high levels of methylglyoxal observed in patients with Type 1 diabetes (McLellan et al. 1994).
Methylglyoxal impairs β-adrenergic signalling in primary rat adipocytes
Published in Archives of Physiology and Biochemistry, 2022
Tomasz Szkudelski, Aleksandra Cieślewicz, Katarzyna Szkudelska
Methylglyoxal (MG) is dicarbonyl aldehyde, which is generated intracellularly predominantly from glucose and fructose. The formation of MG during glycolysis occurs at the level of the 3-carbon glycolytic intermediates. Under physiological conditions, this process is very limited and thereby the spontaneous formation of MG is relatively low (0.05–0.1% of glycolytic flux). However, the enhancement of glycolysis leads to the increased levels of MG (Mey and Haus 2018, Ramachandra Bhat et al.2019). Moreover, apart from endogenous sources, MG may be also provided from some alimentary products, such as honey, cheese and commercial beverages (especially drinks containing fructose) (Tan et al.2008, Gensberger et al.2013, Matafome et al.2017). It is also suggested that harmful effects of food products with higher glycemic index are partially associated with the presence of MG (Adolphe et al.2012, Matafome et al.2017). Therefore, effective degradation of this compound is of high relevance.
Effect of advanced glycation end products on platelet activation and aggregation: a comparative study of the role of glyoxal and methylglyoxal
Published in Platelets, 2021
Cristian Arriagada-Petersen, Paula Fernandez, Maira Gomez, Natalia Ravello, Iván Palomo, Eduardo Fuentes, Felipe Ávila
Bovine serum albumin (1 mM) was incubated with glyoxal (250 mM, BSA-Gly) or methylglyoxal (250 mM, BSA-MG) or without dicarbonyl species (BSA control) for 14 days at pH 7.4 at 37°C in sterile conditions. The concentrations of glyoxal and methylglyoxal were obtained according to the manufacturer’s information. Then samples and controls were exhaustively dialyzed against phosphate buffer 100 mM pH 7.4. After dialysis protein concentration was determined using a BCA assay, according with manufacturer instructions and using a calibration curve build with bovine serum albumin. Samples were stored at −20°C until use.
Transient receptor potential ankyrin 1 (TRPA1)-mediated toxicity: friend or foe?
Published in Toxicology Mechanisms and Methods, 2020
Mohaddeseh Sadat Alavi, Ali Shamsizadeh, Gholamreza Karimi, Ali Roohbakhsh
Methylglyoxal or 2-oxopropanal is a dicarbonyl metabolite that is generated from the glycolysis of triose phosphates (Desai and Wu 2007). Methylglyoxal enhancement has been associated with various diabetes complications including nephropathy and retinopathy (Dhar et al. 2008). Huang et al. showed a relationship between the increase in methylglyoxal level in diabetes and neuropathic pain. Their study demonstrated that administration of STZ in rats raised the methylglyoxal level and induced mechanical allodynia that was prevented by co-injection of a TRPA1 antagonist, A967079 (Huang et al. 2016).