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Oils
Published in Heather A.E. Benson, Michael S. Roberts, Vânia Rodrigues Leite-Silva, Kenneth A. Walters, Cosmetic Formulation, 2019
Fabricio Almeida de Sousa, Vânia Rodrigues Leite-Silva
Alcohols are classified as chemical compounds that have the general formula ROH, where R represents an alkyl group and –OH a hydroxyl group. Depending on how the –OH group is positioned on the alkyl group, alcohols are classified as primary, secondary or tertiary. For example, butanol has three structural isomers – n-butanol (1-butanol or butyl alcohol), 2-butanol (secondary butanol or isobutyl alcohol) and t-butanol (or tert-butyl alcohol: a tert-butanol with a hindered hydroxyl on the same carbon with three methyl groups) (Figure 10.2 ).
Synthesis of novel 6,7-dimethoxy-4-anilinoquinolines as potent c-Met inhibitors
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2019
Qing-Wen Zhang, Zi-Dan Ye, Chang Shen, Hong-Xia Tie, Lei Wang, Lei Shi
The anti-proliferative activities of the prepared compounds (12a-12s) were evaluated against A549, MCF-7 and MKN-45 cell lines by MTT assay in vitro. Briefly, tumour cells were cultured to log phase in RPMI 1640 medium supplemented with 10% foetal bovine serum (FBS). After diluting to 2 × 104 cells mL−1 with the complete medium, 100 µL of the obtained cell suspension was added to each well of 96-well culture plates. Subsequently, incubation was performed at 37 °C in 5% CO2 for 24 h before the anti-proliferative assessment. Tested samples at pre-set concentrations were added to 6 wells with cabozantinib as a positive reference. After 48 h exposure period, 40 µL of PBS containing 2.5 mg mL−1 of MTT was added to each well. Four hours later, 100 µL extraction solution (10% SDS-5% isobutyl alcohol-0.010M HCl) was added. After an overnight incubation at 37°C, the absorbance was measured at a wavelength of 570 nm with an ELISA microplate reader. All compounds were tested three times in each tumour cell lines. The IC50 values were the averages of three determinations and calculated by concentration-response curve fitting method.