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Breast Imaging with Radiolabeled Peptides
Published in Raymond Taillefer, Iraj Khalkhali, Alan D. Waxman, Hans J. Biersack, Radionuclide Imaging of the Breast, 2021
Eric P. Krenning, Marion de Jong, Roelf Valkema, Casper H.J. van Eijck
Thirty minutes after the start of this procedure quality control is performed by instant thin-layer chromatography with silica gel and 0.1 M sodium citrate, pH 5, as eluent. Under these conditions indium citrate and indium chloride migrate along with the solvent front, whereas peptide-bound 111 In stays near the origin.
Analysis of Essential Oils
Published in K. Hüsnü Can Başer, Gerhard Buchbauer, Handbook of Essential Oils, 2020
Adriana Arigò, Mariosimone Zoccali, Danilo Sciarrone, Peter Q. Tranchida, Paola Dugo, Luigi Mondello
For the highly volatile components, commonly present in essential oils, the most adequate transfer technique is partially concurrent eluent evaporation (Grob, 1987). In this technique, a retention gap is installed, followed by a few meters of pre-column and the analytical capillary GC column, both with an identical stationary phase, for the separation of the LC fractionated components. A vapor exit is placed between the pre-column and the analytical column, allowing partial evaporation of the solvent. Hence, column and detector overloading are avoided. This transfer technique can be applied to the analysis of GC components with a boiling point of at least 50°C higher than the solvent. The interface proposed by Grob was improved in 2009 by Biedermann and Grob, introducing the so-called Y-interface in order to reduce the memory effect (Biedermann and Grob, 2009). In the last few years, different transfer devices have been developed to transfer the fractions to the GC system (Purcaro et al., 2013; Biedermann and Grob, 2012).
Antihypertensive effects of oriental drugs in human and SHR
Published in H. Saito, Y. Yamori, M. Minami, S.H. Parvez, New Advances in SHR Research –, 2020
Hideaki Higashino, Aritomo Suzuki, Koichiro Komai
Pheretima aspergillum (E. Perrier) (ref.A, 1990; ref.19a, 1961; ref.19b, 1964). Intravenous or oral administration of tincture, solution, hot dip and decoction of the dry whole body to the anesthetized dogs, rats, cats, and rats with renal hypertension decreased blood pressure mildly but persistently in every case. When it was given to the anesthetized dogs or cats intravenously, acute tachyphylaxis was caused, but the phenomenon wasn’t caused when given orally. The eluent from ion exchangeable resin also decreased prominently in the dogs, rabbits, normal rats and rats with renal hypertension as well as in the cases of the tincture administration. The mechanism of this action was thought to be derived from the dilatation of visceral arteries through a reflexive hypotensive reaction in CNS, though the detail of action is still unclear.
Cannabis-like activity of Zornia latifolia Sm. detected in vitro on rat cortical neurons: major role of the flavone syzalterin
Published in Drug and Chemical Toxicology, 2022
Susanna Alloisio, Marco Clericuzio, Mario Nobile, Annalisa Salis, Gianluca Damonte, Claudia Canali, Ana Paula Fortuna-Perez, Laura Cornara, Bruno Burlando
Selected samples were analyzed in an Agilent 1100 HPLC-MSD Ion Trap XCT system, equipped with an electrospray ion source (HPLC-ESI–MS) (Agilent Technologies, Palo Alto, CA, USA) in negative ion mode. Separations were performed on a Jupiter C18 column 1 × 150 mm with 5-μm particle size (Phenomenex Torrance, CA, USA). Eluents were water (eluent A) and CH3CN/CH3OH (eluent B), both added with 0.1% formic acid. The gradient employed was: 30% eluent B for 5 min, linear to 85% eluent B in 12 min, then linear to 95% in 3 min, and finally hold at 95% eluent B for other 5 min. The flow rate was set to 30 μL/min and the column temperature was set at 30 °C. The injection volume was 8 μL. Ions were detected in ion charged control with a target ion value of 200 000 and an accumulation time of 300 ms, using capillary voltage: 3300 V; nebulizer pressure: 15 psi; drying gas: 8 L/min; dry temperature: 325 °C; rolling averages: 2; averages: 5. Mass spectra were acquired in the negative ion mode in a range of 100–500 m/z consistent with expected mass/charge ratios. MS2 analysis was conducted using fragmentation amplitude at 0.9 V.
A novel multi-stimuli-responsive theranostic nanomedicine based on Fe3O4@Au nanoparticles against cancer
Published in Drug Development and Industrial Pharmacy, 2020
Bakhshali Massoumi, Amir Farnudiyan‐Habibi, Hossein Derakhshankhah, Hadi Samadian, Rana Jahanban-Esfahlan, Mehdi Jaymand
The initiator was synthesized through the esterification of bis(2-aminoethyl) disulfide hydrochloride using BIBB in the presence of NEt3 as HBr and HCl scavenger as follows. A 100-ml two-neck round-bottom glass reactor was equipped with a condenser, a dropping funnel, and a magnetic stirred. The reactor was charged with bis(2-aminoethyl) disulfide hydrochloride (1.00 g, 4.5 mmol), NEt3 (2.8 ml, 20 mmol), and derided CH2Cl2 (50 ml). The content of the reactor was cooled to 4 °C using an ice/water bath, and then a CH2Cl2 solution of BIBB (1.1 ml, 9 mmol; in 30 ml of derided CH2Cl2) was added dropwise into the reactor. At the end of this time, the temperature allowed to increase to the room temperature, and the reaction mixture was stirred for about 5 h. Afterward, the crude product was extracted with distilled water for three times, in order to remove unreacted reagents as well as by-products. The product was further purified using a short column chromatography by n-hexane/ethyl acetate (1/1 v/v) as the eluent.
Synthesis, purification, and anticancer effect of magnetic Fe3O4-loaded poly (lactic-co-glycolic) nanoparticles of the natural drug tetrandrine
Published in Journal of Microencapsulation, 2019
Kaiping Wang, Huiping Hu, Qian Zhang, Yu Zhang, Chen Shi
MACS® technology was applied to purify the co-loaded PLGA NPs due to their special magnetic properties (Shinkai 2002; Dunnill and Lilly 1974). The dynabeads filling inside the separation column showed strong superparamagnetism when placed in a permanent magnetic field. When the co-loaded PLGA NPs were passed through the MACS magnetic separation column, these NPs could be effectively combined with the dynabeads. However, the free Tet and Tet-PLGA NPs without magnetic materials loaded inside would be directly eluted by the deionised water. When the permanent magnetic field was removed, the dynabeads did not retain the magnetism and lost the ability to combine with the co-loaded PLGA NPs. Washed the column with deionisation water for three times and collected the eluent. Then, the purified co-loaded PLGA NPs were obtained.