China
Africa
Explore chapters and articles related to this topic
Bleomycin Assay for Catalytic Iron Salts in Body Fluids
Published in Robert A. Greenwald, CRC Handbook of Methods for Oxygen Radical Research, 2018
John M. Gutteridge, Barry Halliwell
Bleomycin sulfate is dissolved to 1 mg/mℓ in pyrogen-free water. This solution is not treated with Chelex 100, as the resin appears to bind bleomycin. Take care when opening sealed ampules not to inhale the dust.
Image processing techniques and orthodontic related apical root resorption prediction models
Published in R.M. Natal Jorge, J.C. Reis Campos, Mário A.P. Vaz, Sónia M. Santos, João Manuel R.S. Tavares, Biodental Engineering IV, 2017
S. Alves, H. Silva, L. Mesquita, N. Lavado, M. Lopez
DNA was extracted from buccal swabs using Chelex 100® (Sigma-Aldrich, St Louis, MO, USA). Four single-nucleotide polymorphisms (SNPs) were assessed: rs1718119 from the gene encoding the purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), rs1143634 from interleukin 1B (IL1B) gene, rs3102735 from osteoprotegerin (OPG/TNFRSF11B) gene and rs1805034 from the gene encoding the receptor activator of nuclear factor-κB (RANK/TNFRSF11A). Genotyping was performed by restriction fragment length polymorphism (RFLP) assays, real time Taq-Man assays and Sanger sequencing, as previously described (Pereira et al., 2014).
Methods for Genetic Testing I
Published in Peter G. Shields, Cancer Risk Assessment, 2005
Haruhiko Sugimura, Peter G. Shields
Other variables that affect DNA quality and quantity occur during DNA extraction, such as insufficient time for dewaxing with xylene, detergent (SDS or tween-20) or insufficient time for proteinase K digestion. Greater quantities of the paraffin embedded tissue are not necessarily better for DNA assays, because this allows for greater amounts of inhibitors from the blocks. Several agents are available for extracting DNA from fixed tissues that are thought to reduce these inhibitors, e.g., Chelex-100 (22).
Genetic polymorphisms of 19 X-STRs in populations of Hubei Han and Guangxi Zhuang and their comparisons with 13 other Chinese populations
Published in Annals of Human Biology, 2023
Fei Long, Hui Fang, Chunmei Zhang, Shengjie Chen, Daixin Huang, Chao Xiao
DNA extraction, PCR, and fragment analysis were performed as described by Xiao et al. (2020). In brief, genomic DNA was isolated from the samples using the Chelex-100 method. Multiplex PCR was then performed using an AGCU X19 STR kit (AGCU ScienTech, Wuxi, China) according to the manufacturer’s recommendations in a 2720 thermal cycler (Applied Biosystems, CA, USA). PCR products were subsequently separated through capillary electrophoresis on an ABI 3130 Genetic Analyser and finally analysed using GeneMapper v3.2 software (Applied Biosystems, CA, USA). Bidirectional Sanger sequencing was used to analyse allelic dropouts at the DXS10164 locus using the procedure described by Yu et al. (2016). The primers for PCR and Sanger sequencing were as follows: 5′-GCC TAG GCA AGT TTT CAA GAG TAG-3′ (Forward) and 5′-CCT AAA CAA CCA AAG CAA CTC AAC-3′ (Reverse).
Gene flow and phylogenetic analyses of paternal lineages in the Yi-Luo valley using Y-STR genetic markers
Published in Annals of Human Biology, 2021
Guang-Yao Fan, Dan-Lu Song, Hai-Ying Jin, Xing-Kai Zheng
DNA samples of 2,314 unrelated healthy male inhabitants from seven locations of the Yi-Luo valley, Yanshi (N = 129), Xin'an (N = 316), Luanchuan (N = 199), Songxian (N = 337), Yiyang (N = 167), Luoning (N = 451), and Yichuan (N = 715), were collected on buccal swabs (Supplementary Table S1). Genomic DNA was extracted using the Chelex-100 method. The household registration records were used to confirm Han nationality identity to others. All the participants were residents in the selected locations for at least three generations. Informed consent was obtained from all participating subjects. Ethical permission for recruitment and analysis was provided by the Ethics Committee of the Medical College, Shaoxing University (2020–001).
Updated population genetic data of 15 autosomal STR loci in a Shandong Han population from East China and genetic relationships among 26 Chinese populations
Published in Annals of Human Biology, 2020
Li Luo, Hongyan Gao, Lilan Yao, Haidong Liu, Hao Zhang, Jian Wu, Guanglin He, Pengyu Chen
A total of 5356 unrelated healthy Han people, whose ancestors have resided in Shandong Province from East China for at least 3 generations, were investigated. The project was conducted in accordance with the guidelines approved by the Ethics Committee of the Zunyi Medical University. Each participant signed informed consent. Blood samples spotted on the Flinders Technology Associates (FTA) cards (Whatman, UK), were collected from the routine cases of personal identification and paternity testing, during the period 2015–2017. Genomic DNA was extracted from Chelex 100 described by Walsh et al. (2013). To explore the population genetic relationships between our studied population and other reported populations, 10 Han populations (Huang et al. 2013; Wang et al. 2014; Ruan et al. 2015; Xiang et al. 2016; Yao et al. 2016; Chen et al. 2017; Sun et al. 2017; Yao et al. 2017; Zou et al. 2017; Li et al. 2018) and 15 minority populations (Liu et al. 2013; Yuan et al. 2014; Zhang 2015a, 2015b; Sun et al. 2016; Jin et al. 2017; Ma et al. 2017; Zhang et al. 2017; Feng et al. 2018; Li et al. 2018; Fan et al. 2019) from Guizhou and other regions (Supplementary Figure S1) were selected.