China
Africa
Explore chapters and articles related to this topic
Preparation and Health Benefits of Rice Beverages From Ethnomedicinal Plants: Case Study in North-East of India
Published in Megh R. Goyal, Arijit Nath, Rasul Hafiz Ansar Suleria, Plant-Based Functional Foods and Phytochemicals, 2021
Vedant Vikrom Borah, Mahua Gupta Choudhury, Probin Phanjom
Biuret test is used as a standard method for the qualitative detection of protein in rice beer. Bovine serum albumin is used as a standard in Biuret test [13, 39, 40]. Reports suggest the presence of protein with concentration of 0.97 mgmL−1 in judima [39], 1.05 mgmL−1 in poro apong [40], 9.63-12.42 mgmL−1 in rice beer prepared by tribes in Tripura [13], 3.2 mgmL−1 in boro, 5.7 mgmL−1 in poro, 4.2 mgmL−1 in judima and 6.2 mgmL−1 in jumai [43].
Toxicity and Chosen Behavioral and Physiological Effects of Some Tin Steroid Compounds on Selected Insect Species*
Published in Nate F. Cardarelli, Tin as a Vital Nutrient:, 2019
R. N. Sharma, Vrushali Tare, S. B. Bhonde
In both cases, two weighed larvae were homogenized in 2 mℓ of distilled water. Amount of protein present was calculated by subjecting the homogenates to the Biuret test procedure of Gornall (1949). Bovine albumin powder (Frn. V) from bovine serum was used to build the standard protein curve, from which the whole body total protein contents of the larvae were obtained by extrapolation.
Statistical development and in vivo evaluation of resveratrol-loaded topical gel containing deformable vesicles for a significant reduction in photo-induced skin aging and oxidative stress
Published in Drug Development and Industrial Pharmacy, 2020
Daisy Arora, Bharat Khurana, Sanju Nanda
Treated skin samples of animals of each group were harvested using the method given in the previous section. The processing of skin samples was done according to reported methods [30]. The excised skin was rinsed with cold normal saline and stored in ice-cold 1.15% w/w KCl solution after chopping it in small pieces until further use. Then it was homogenized with a tissue homogenizer (WiseMix HG-15D) for 20 min to get a thick viscous mass free from solid or suspended particles. The homogenized mass was then centrifuged for 15,000 g for 15 min and the supernatant fraction was collected. It was stored in the deep freezer till further use and the temperature was maintained below 4 °C at all the stages of processing to avoid the production of free radicals and degradation of proteins and other enzymes. This supernatant was used for the estimation of various endogenous oxidative enzymes and protein content as biomarkers of skin aging. The levels of first-line defense antioxidants superoxide dismutase (SOD) [30,34], catalase [35], and glutathione [36] were estimated using specific reported methods. The extent of lipid peroxidation was estimated by determining the levels of malondialdehyde (MDA) a secondary product of lipid peroxidation (indirect index) as reported [37]. The content of elastin was determined using a commercial mouse elastin Elisa Kit [38]. Total protein content in skin homogenates was analyzed using the Biuret test [31].
Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation: in vitro and in vivo study
Published in Drug Development and Industrial Pharmacy, 2018
Vandita Kakkar, Indu Pal Kaur, Amrit Pal Kaur, Komal Saini, Kamalinder K. Singh
At the end of 14th day, the mice were sacrificed by ether inhalation. Mice skin was excised and rinsed in ice cold phosphate buffer saline (pH 7.4). The homogenate of the skin 8% (g of skin in volume of phosphate buffer saline) was prepared using tissue homogenizer at 5000 rpm. It was then, centrifuged at 10,000 rpm at 4 °C for 10 min and the clear supernatants were collected and used for the following biochemical estimations of various groups. Protein estimation was performed using biuret test [35]. Lipid peroxidation, reduced glutathione estimation, superoxide dismutase (SOD) and catalase activity was performed according to the standard referred methods [36,37].
Studies on pomegranate seed oil enriched galantamine hydrobromide microemulsion: formulation, in vitro antioxidant and neuroprotective potential
Published in Pharmaceutical Development and Technology, 2023
Meenakshee Shrivas, Dignesh Khunt, Meera Shrivas, Manju Misra
The total protein content of each lysate was determined by the Bicinchonic acid (BCA) assay (which utilizes the biuret test). Ten times diluted lysate was transferred into 96 well plates in triplicates and 200 µL BCA reagent was added to it. The reaction mixture was incubated at 37 °C for 30 min and absorbance was taken at 562 nm wavelength. Finally, the protein content was estimated using a calibration curve previously prepared (He 2016).