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Analytical Toxicology of Vitreous Humor
Published in Steven H. Y. Wong, Iraving Sunshine, Handbook of Analytical Therapeutic Drug Monitoring and Toxicology, 2017
Detection of barbiturates in vitreous humor was reported as early as 1969, by Felby and Olsen.10 Using the classic technique of recording ultraviolet absorption after pH adjustment, the presence and quantity of barbiturates was determined in blood, blood subjected to ultrafiltration, vitreous humor, and liver. They found a good correlation between the ultrafiltrate of blood and vitreous humor, but no correlation was detected when comparing whole blood and vitreous humor. Based on the finding of nearly equivalent concentrations of barbiturate in ultrafiltrate of blood and in vitreous humor, it was presumed that, in each of these cases, death had been delayed more than 5 hours after ingestion of the drug. Meprobamate was also determined in three cases by Felby and Olsen,10 using spectrophotometric analysis after development of a chromophore with p-dimethylaminobenzalde-hyde and antimony trichloride. The results showed similar equivalence of concentrations in blood, vitreous humor, and ultrafiltrates of blood.
In vivo evaluation of antivenom activity of Adenocarpus anagyrifolius methanolic extract against Hottentotta gentili scorpion venom
Published in Toxin Reviews, 2023
Karima Benrazzouk, Mehdi Ait Laaradia, Malika Ait Sidi Brahim, Soukaina Ouhaddou, Ahmed Ouhammou, Abderrahman Chait, Khalid Bekkouche, Mohammed Markouk, Mustapha Larhsini
One gram of the powdered plant material was extracted with 10 ml of 60% ethanol in an ultrasonic bath for 30 min. The extract was then filtered and evaporated to dryness using a rotary evaporator and the final residue was taken up in 1 ml of methanol. Layer chromatography was performed by a few µl of extract obtained. The migration solvent used was 100% ethyl acetate. After migration, the TLC plate was observed at 254 and 365 nm, then sprayed with antimony trichloride. Any blue fluorescence indicates the presence of coumarins after UV examination at 365 nm.