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Order Cirlivirales
Published in Paul Pumpens, Peter Pushko, Philippe Le Mercier, Virus-Like Particles, 2022
Paul Pumpens, Peter Pushko, Philippe Le Mercier
Tao et al. (2020) elaborated another bivalent vaccine based on the nonchimeric PCV2 VLPs and a recombinant chimera of three antigens from Mycoplasma hyopneumoniae that is also an economically important pathogen of swine. The recombinant proteins were efficiently produced in E. coli, and the combined immunization with them induced strong humoral and cellular immune responses against all four antigens in mice and piglets.
Animal diseases
Published in Jim Cox, Iain Mungall, Rural Healthcare, 2017
Pig farmers try to achieve ‘minimal disease status’, but problems such as enzootic pneumonia caused by Mycoplasma hyopneumoniae are difficult to eliminate. Sometimes it is necessary to cull all the sows and give a house a period of rest. However, even if replacement gilts (young sows) are obtained from so-called ‘disease-free status’ herds, infections such as enzootic pneumonia often return within a year or two.
Nuclease activity: an exploitable biomarker in bacterial infections
Published in Expert Review of Molecular Diagnostics, 2022
Javier Garcia Gonzalez, Frank J. Hernandez
In fact, recent studies have identified membrane-associated extracellular nucleases in Mycoplasma meleagridis (Mm19) [83] and Mycoplasma hyopneumoniae (mhp379) [84], common animal pathogens, as well as in species isolated from humans, such as Mycoplasma pneumoniae (M. pneumoniae) (Mpn133) [85], Mycoplasma genitalium (MG-168) [86] or Mycoplasma penetrans (P40) [87,88]. Interestingly, mhp379, MG-168 and Mpn133 are Ca2+-dependent nuclease homologues with broad substrate specificity belonging to the MN cluster of orthologous proteins (COG1525) that are encoded upstream of genes encoding for homologous ABC transport systems, which is in line with the previously proposed nutrient scavenging roles of mycoplasma nucleases [28]. This hypothesis is further supported by the ability of other mycoplasmas to use undegraded DNA and RNA as a nutrient source [89] and the observed reduction of the cytotoxic effects of M. pneumoniae in the presence of adenine supplement [90]. However, their ability to induce internucleosomal DNA degradation [86,91,92] and observations in in vitro models of human-derived cell of membrane binding, internalization, reduction of viability and induction of apoptosis [85,88], also suggest a role of mycoplasmas´ extracellular nucleases as pathogenic determinants.
Thirty years of recombinant BCG: new trends for a centenary vaccine
Published in Expert Review of Vaccines, 2021
Lazaro M Marques-Neto, Zuzanna Piwowarska, Alex I. Kanno, Luana Moraes, Monalisa M Trentini, Dunia Rodriguez, Jose L. S. C. Silva, Luciana C. C. Leite
Moreover, several studies have shown adjuvant properties for rBCG strains expressing bacterial antigens. The expression in rBCG of the Mycoplasma hyopneumoniae antigen in fusion with the B subunit of the heat labile enterotoxin (LTB) doubled the titers of specific IgG and IgA as compared to the antigen alone [74]. Likewise, intranasal administration of rBCG expressing the B subunit of cholera toxin (CTB) increased the response to OVA (ovalbumin), indicating its potential as a mucosal adjuvant [76]. Additionally, the combination of rBCG strains expressing the diphtheria antigen (CRM197) and tetanus toxin fragment C (FC) demonstrated a reciprocal increase in immune responses as compared to each strain alone [77].
Device safety assessment of bronchoscopic microwave ablation of normal swine peripheral lung using robotic-assisted bronchoscopy
Published in International Journal of Hyperthermia, 2023
Hector De Leon, Kevin Royalty, Louie Mingione, David Jaekel, Sarvesh Periyasamy, David Wilson, Paul Laeseke, William C. Stoffregen, Tim Muench, John P. Matonick, Grzegorz L. Kaluza, Gustavo Cipolla
Coughing exhibited by a 3-Day (ID: 7) and a 30-Day (ID:13) animal was classified as a minor AE (SIR level A) since both animals fully recovered without therapy (Table 1). However, a 30-Day swine (ID: 15) with recurrent episodes of coughing required antibiotic therapy and was thus classified as a major AE (C). The animal fully recovered two weeks later. The 3 swine that exhibited coughing (one 3-Day and two 30-Day animals) were positive for Mycoplasma hyopneumoniae. Therefore, 3 out of 17 animals (18%) exhibited clinical signs consistent with mycoplasma pneumonia confirmed by molecular testing (Table 1).