Leucyl aminopeptidase

Tonic Influences on Baroreceptor Reflex Sensitivity By Endogenous Neurotensin in the Nucleus Tractus Solitarii

Published in I. Robin A. Barraco, Nucleus of the Solitary Tract, 2019

Along with being a specific arginyl aminopeptidase and leucine aminopeptidase inhibitor,16-18 bestatin may also be a potent blocker of alanyl and leucyl aminopeptidase.16 Based on these properties and presently available data on amino acid sequences and peptide degradation, it follows that the neuropeptides most likely to be protected by this degradative enzyme blocker may at least include, albeit not exclusively, neurotensin, angiotensin III, substance P, and somatostatin.

Melatonin modulates oxidative phosphorylation, hepatic and kidney autophagy-caused subclinical endotoxemia and acute ethanol-induced oxidative stress

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Journal Information

Published in Chronobiology International, 2020

Natalia Kurhaluk, Halyna Tkachenko, Oleksandr Lukash

The activity of alanyl aminopeptidase (EC 3.4.11.2) and leucyl aminopeptidase (EC 3.4.11.1) was determined spectrophotometrically as Fast Blue BB salt (4-benzoyloamino-2,5-diethoxybenzene-diazonium chloride) derivatives at 540 nm according to McDonald and Barrett (1986). The reaction was initiated by mixing 50 μl of sample and 500 μl of substrate incubation media with DMF (Serva, Germany) for 60 min incubation at 37°C, pH 6.0. Next, 500 μl of stop buffer containing Fast Blue BB Salt dissolved in 2% Tween 20 (Sigma, USA) was added, and measurements were carried out at 540 nm. For determination of alanyl aminopeptidase activity, L-alanyl-2naphtylamine in 0.1 M PBS buffer was used as a substrate. L-leucyl-2-napthylamine in 0.1 M PBS (pH 7.0) buffer was used as a substrate for determination of leucyl aminopeptidase activity.

Leucyl aminopeptidase

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Tonic Influences on Baroreceptor Reflex Sensitivity By Endogenous Neurotensin in the Nucleus Tractus Solitarii

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