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Sleep
Published in Carolyn Torkelson, Catherine Marienau, Beyond Menopause, 2023
Carolyn Torkelson, Catherine Marienau
Judy, a 60-year-old attorney, came into my office because of heartburn and a skin rash around her pant line at the waist. She had been working on a big case and found herself becoming very irritable and unable to sleep. Her partner commented that her shift in behavior was affecting their relationship. Judy had seen an Ayurvedic practitioner in the past and was familiar with Ayurvedic principles. Although I am not an Ayurvedic practitioner, I was able to see by her physical appearance that she had an imbalance in the Pitta dosha with too much fire and heat. I suggested that she cool down her Pitta dosha and balance out her excess fire. She acknowledged that her busy work schedule had kept her out of touch with her body, and she needed a reminder to revisit “cooling” strategies. She agreed that a revisit with her Ayurvedic practitioner was needed, but she could do some self-treatment in the meantime. We discussed the need to slow down and eat three meals, with the biggest meal being at noon, and to take a relaxing cooling bath before bedtime to help improve sleep.
Preparation of Samples for Liquid Scintillation and Gamma Counting
Published in Howard J. Glenn, Lelio G. Colombetti, Biologic Applications of Radiotracers, 2019
Oxygen flask method (static) — The oxygen flask method as developed by Schoniger,29 and modified,30 is simple and convenient. It does not require the use of elaborate or expensive equipment. It consists primarily of a 2- or 3-ℓ flask, as shown in Figure 4.8 The sample is dried in a plastic sheet or filter paper container and placed in the sample carrier. The flask is then purged of air, filled with oxygen, and closed. The sample is ignited by means of a spark or by a focused infrared light source. The trapping agent is added and the flask shaken until the carbon dioxide is absorbed. If the trapping agent is added prior to combustion, the flask is immersed in a dry ice-acetone bath to prevent ignition of the trapping agent; the sample is then ignited, and when combustion is complete, the flask is removed from the cooling bath and shaken until the carbon dioxide is absorbed. Although the unit will take only small samples, and sometimes the dissolved oxygen causes quenching, the method works well when only a limited number of samples need processing. Isotope recovery is about 96%.
Tissue Preparation for Liquid Scintillation and Gamma Counting — the Counting Processes
Published in Lelio G. Colombetti, Principles of Radiopharmacology, 2019
Howard J. Glenn, Lelio G. Colombetti
The oxygen flask method — The oxygen flask method as developed by Schöniger,24 and as modified by many, is simple and convenient. It does not require the use of elaborate or expensive equipment. It consists primarily of 2- or 3-ℓ flask, as shown in Figure 6.9 The sample is dried in a plastic sheet or filter paper container and placed in the sample carrier. The flask is then purged of air, filled with oxygen, and closed. The sample is ignited by means of a spark or by a focused infrared light source. The trapping agent is added and the flask shaken until the carbon dioxide is absorbed. If the trapping agent is added prior to combustion, the flask is immersed in a dry ice-acetone bath to prevent ignition of the trapping agent, the sample is then ignited, and when combustion is complete, the flask is removed from the cooling bath and shaken until the carbon dioxide is absorbed. Although the unit will take only small samples, and sometimes the dissolved oxygen causes quenching, the method works well when only a limited number of samples need processing. Isotope recovery is about 96%. A commercial model employing spark combustion, water condensation, and carbon dioxide trapping permitting direct drainage into scintillation counting vials is available as the 6550 system (Searle Analytic).25
Design, synthesis, apoptotic, and antiproliferative effects of 5-chloro-3- (2-methoxyvinyl)-indole-2-carboxamides and pyrido[3,4-b]indol-1-ones as potent EGFRWT/EGFRT790M inhibitors
Published in Journal of Enzyme Inhibition and Medicinal Chemistry, 2023
Lamya H. Al-Wahaibi, Anber F. Mohammed, Fatema El-Zahraa S. Abdel Rahman, Mostafa H. Abdelrahman, Xuyuan Gu, Laurent Trembleau, Bahaa G. M. Youssif
A solution of indole ester 1 (1 g, 3.97 mmol) in DMF (0.2 M) was added dropwise at 0 °C to a suspension of NaH (0.25 g, 5.96 mmol, 60% dispersion in mineral oil) in DMF (0.2 M). After stirring 0.5 h, the resulting mixture was treated dropwise with (Boc)2O (1.34 g, 5.96 mmol). The cooling bath was removed, and the mixture was stirred overnight at rt. The reaction mixture was diluted with EtOAc and successively washed twice with water. EtOAc layer was washed with brine, dried over MgSO4, and concentrated under reduced pressure to yield compound 2 (1.1 g, 71%) as a white solid after purification by flash chromatography on silica gel using a mixture of EtOAc, hexanes (1:10) as eluent; mp 58–59 °C.
Acoustic emission detection of crystallization in two forms: monohydrate and anhydrous citric acid
Published in Pharmaceutical Development and Technology, 2019
Xingjun Wang, Ying Huang, Thomas M. Michelitsch
A schematic of the crystallization setup used during the present study is displayed in Figure 3. A 2 L glass vessel equipped with a jacket which was baffled and the pump of the cooling bath forced the circulation. Cooling was performed by means of heat transfer through the jacket wall. Stainless-steel baffles were used to avoid vortex formation and a high efficiency propeller (Mixel TT TM, Lyon, France) was set in the crystallizer with a rotational speed of 300 rpm/min across all experiments so as to maintain a good homogeneity of the particles in suspension. The end particle was analyzed by µ-Raman spectroscope (Jobin Yvon xplora, Paris, France) to confirm its form.
Identification and quantification of isoguanosine in humans and mice
Published in Scandinavian Journal of Clinical and Laboratory Investigation, 2019
Allan Weimann, George McLeod, Trine Henriksen, Vanja Cejvanovic, Henrik E. Poulsen
The column, the injection volume and the gradient were adjusted depending on the matrix analyzed (see Tables 1–3). The column was immersed in a cooling bath with a temperature of 1 °C. This temperature was chosen because nucleosides are better retained at low temperatures. Thus, it was possible to focus the nucleosides on top of the column before the elution started, enabling the use of large injection volumes without generating broad peaks [28]. During chromatography, it is especially important to ensure that adenosine does not co-elute with isoguanosine because a small fraction of adenosine can become oxidized in the ion source and spuriously form either isoguanosine or 8-oxo-7,8-dihydroadenosine (8-oxoAdo), which could bias or interfere with isoguanosine measurements. It seems, however, that less than 1/10,000 adenosine molecules are oxidized in this way, so the separation is expected to only really be critical in tissue samples with an overwhelming amount of unmodified adenosine. Initially when measuring the adenosine and isoguanosine content in mouse liver tissue, the method shown in Table 3 was used directly on the hydrolyzed extracts, but here adenosine and isoguanosine elutes close to one another. To make sure that the isoguanosine that was measured was not artificially formed by oxidation in the ion source, the identification of isoguanosine in the hydrolyzed RNA extracts was confirmed by first pre-fractionation by the same UPLC method as used for the pre-fractionation (separation) of adenosine and isoguanosine in urine (see Table 6). After this the identity of isoguanosine was confirmed by analyzing the collected fractions by the method shown in Table 3. The levels of isoguanosine measured by both the direct method and after pre-fractionation were identical.