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A Pilot Study in Non-Human Primates Shows No Adverse Response to Intravenous Injection of Quantum Dots
Published in Lajos P. Balogh, Nano-Enabled Medical Applications, 2020
Ling Ye, Ken-Tye Yong, Liwei Liu, Indrajit Roy, Rui Hu, Jing Zha, Hongxing Cai, Wing-Cheung Law, Jianwei Liu, Kai Wang, Jing Liu, Yaqian Liu, Yazhuo Hu, Xihe Zhang, Mark T. Swihart, Paras N. Prasad
Chloroform dispersions of quantum dots (∼4 mgml−1) and of DSPE-mPEG (Avanti Polar Lipids or Laysan Bio 1,2-diacyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)], ∼10 mg ml−1) were mixed in a 1:4 mass ratio and gently stirred for 5–10 min. A Labconco rotary evaporator with a water bath at 25◦C was used to evaporate the chloroform. The resulting lipidic film was hydrated with 3–5 ml of high-pressure liquid chromatography grade water and sonicated for 10–15 min using a bath sonicator. The resulting dispersion was filtered through a 0.45 or 0.2 µm membrane filter and kept at room temperature for further use. To remove excess phospholipid, the micelle-encapsulated quantum dots were centrifuged at 10,000 r.p.m. for 15 min. The precipitate was then redispersed in 1–2 ml of HPLC water.
ASSESSMENT OF ANTICANCER AND IMMUNOMODULATORY ACTIVITY OF Asystasia travancorica Bedd. (ACANTHACEAE)
Published in V. R. Mohan, A. Doss, P. S. Tresina, Ethnomedicinal Plants with Therapeutic Properties, 2019
P. S. Tresina, K. Paulpriya, V. Sornalakshmi, V. R. Mohan
The dried whole plant material of A. travancorica was powdered in a Wiley mill. Hundred grams of whole plant powder was packed in a Soxhlet apparatus and extracted with ethanol. The ethanol extract was concentrated in a rotary evaporator. The concentrated ethanol extract was used for anticancer and immunomodulatory studies.
Black Cumin: A Review of Phytochemistry, Antioxidant Potential, Extraction Techniques, and Therapeutic Perspectives
Published in Megh R. Goyal, Durgesh Nandini Chauhan, Plant- and Marine-Based Phytochemicals for Human Health, 2018
Muhammad Jawad Iqbal, Masood Sadiq Butt, Hafiz Ansar Rasul Suleria
Traditionally, different solvents and extraction techniques are used for extraction of active components. One of these techniques includes Soxhlet extraction followed by rotary evaporator for removal of chemical solvents. The technology of Soxhlet extraction and Soxhlet extraction apparatus are detailed in: [http://nptel.ac.in/courses/102103016/module4/ lec33/images/1.jpg].
Utilization of a nanostructured lipid carrier encapsulating pitavastatin–Pinus densiflora oil for enhancing cytotoxicity against the gingival carcinoma HGF-1 cell line
Published in Drug Delivery, 2023
Raed I. Felimban, Hossam H. Tayeb, Adeel G Chaudhary, Majed A. Felemban, Fuad H. Alnadwi, Sarah A. Ali, Jazia A. Alblowi, Eman ALfayez, Deena Bukhary, Mohammed Alissa, Safa H. Qahl
A previously published hot homogenization and sonication method was adopted for the development of the different PV-Pd-NLCs formulations (Chauhan et al., 2020). In brief, predetermined quantities of Ovucire®, Pd oil, Capryol 90®, and PV (10 mg), which comprised the lipid phase, were dissolved in a 1:1 mixture of chloroform and methanol. A rotary evaporator (R 10 BÜCHI Labortechnik AG, Flawil, Switzerland) was used to eliminate the organic solvent mixture. Then, the drug’s lipid layer was melted at 65–70 °C. In the meantime, predefined amounts of Labrasol® were dissolved in distilled water and then this aqueous phase was warmed at 65–70 °C. The warmed aqueous phase was decanted into the lipid phase, and the mixture was homogenized (IKA Homogenizer T18; IKA, Karnataka, India) at 6,000 to 24,000 rpm for 2 min. The developed emulsion was sonicated using a probe sonicator (Sonics Vibra-Cell VCX 750; Sonics and Materials, Inc., Newtown, CT, USA) for 1 min, and the NLCs that resulted were kept at 25 °C. The prepared PV-Pd-NLCs were lyophilized (Martin Christ GmbH, Osterode am Harz, Germany) at a condenser temperature of –45 °C and pressure of 7 × 10−2 mbar using mannitol as a cryoprotectant for 24 h and kept for further evaluation.
Effect of PEGylation on drug uptake, biodistribution, and tissue toxicity of efavirenz–ritonavir loaded PAMAM G4 dendrimers
Published in Pharmaceutical Development and Technology, 2023
Rohini Kharwade, Nilesh Mahajan, Sachin More, Amol Warokar, Sachin Mendhi, Akshay Dhobley, Devendra Palve
RP-HPLC: LC-20 AD was obtained from Shimadzu (Kyoto, Japan). UV spectrophotometer: UV-1800 was obtained from Shimadzu (Kyoto, Japan). Rotary evaporator was obtained from Buchi Rotavapor R-100 (Buchi, Flawil, Switzerland). Cooling Centrifuge was obtained from REMI Model CM 12 Plus (REMI, Mumbai, India). Lyophilizer was obtained from MAC Lyophilizer (Freeze Dryer) (cat. no.: MSW-137) serial no. 2511 was obtained from Macro Scientific Works Pvt. Ltd. (Delhi, India). Mass spectrophotometer: Synapt XS HDMS, UPLC Acquity H class series system was obtained from Waters (Wilmslow, UK). FE-SEM: JSM-6380A Scanning Electron Microscope; 200 kV was obtained from JEOL Ltd. (Tokyo, Japan). TEM: JEM-2100 Plus Electron Microscope; 200 kV was obtained from JEOL Ltd. (Tokyo, Japan). DSC: Mettler Toledo, DSC1 was obtained from Star e-software (Ramsen, Switzerland). XRD: powder X-ray diffractometer with Ni-filtered, Bruker D2 phaser, 2nd generation was obtained from Bruker (Karlsruhe, Germany). CO2 incubator was obtained from Thermo Scientific Inc. (Waltham, MA). ELISA microplate reader: Benesphera E2 (110–250 V; OSRAM 64607) was obtained from Benesphera (Center Valley, PA). Rotary microtome: Leica Biosystem, Semiautomatic Histocore Rotary Microtome; was obtained from Leica Biosystem (Wetzlar, Germany). Optical microscope: Cilika BT-E (2021); Benchtop Biological Digital microscope was obtained from Medprime Technology Private Ltd. (India).
D-optimal mixture design for optimization of topical dapsone niosomes: in vitro characterization and in vivo activity against Cutibacterium acnes
Published in Drug Delivery, 2022
Basant A. Habib, Nourtan F. Abdeltawab, Ibtehal Salah Ad-Din
Dapsone niosomes were prepared by the conventional thin film hydration sonication method reported by Bangham et al. (1965) with slight modifications. Specific amounts of Span 20, Cholesterol, Cremophor RH, and drug were dissolved in 9 mL of 2:1 methanol: chloroform mixture using bath sonicator (Crest Ultrasonics Corp., Trenton, USA) for 3 min. The resultant solution was then transferred to the 1-L round bottomed flask of a rotary evaporator (Rotary evaporator RE300P, Stuart, Staffordshire, UK) for evaporation of solvents under vacuum. Evaporation under vacuum lasted for 10 min at 60 °C. The residual film was then hydrated to 10 mL using double distilled water containing 0.01% sodium lauryl sulfate by rotation at 60 °C for 20 min under atmospheric pressure. The prepared niosomal suspension was then sonicated for 5 min for size reduction (Habib et al., 2018). All the prepared niosomes were then stored in the refrigerator (4 °C) for 24 h before further characterization.