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B Cells and Immunoglobulins
Published in Miroslav Holub, Immunology of Nude Mice, 2020
The antibacterial response (Brucella abortus) consisted in nu/nu of IgM antibodies and was considerably prolonged over the control (BALB/c) response.67Bordetella pertussis had only a limited adjuvant effect on the primary response to SRBC and the background PFC, a slight increase of IgG PFC occurred in the primary response but no secondary response could be incuded in NMRI nude mice of uncertain health status.68 Gnotobiotic nu/nu mice recovering from Campylobacter infection were found to produce specific antibodies of the IgG class.70a An IgG response could be produced in BALB/c nudes also by mucosal stimulation with Mycoplasma pulmonis.71a
Laboratory Diagnostic Tests in the Evaluation of Fever
Published in Benedict Isaac, Serge Kernbaum, Michael Burke, Unexplained Fever, 2019
Antibody against Brucella is detected through a simple tube agglutination test which employs a standard antigen from Brucella abortus. The test is sufficient for detection of antibody to other Brucella species, with the exception of Brucella canis. False-positive titers have been described in tularemia, typhoid fever, and yersiniosis.
Chemistry of Lymph
Published in Waldemar L. Olszewski, Lymph Stasis: Pathophysiology, Diagnosis and Treatment, 2019
Subcutaneous injection of Brucella abortus organisms resulted in an increase in IgG and IgM concentrations in efferent lymph that reached a peak on days 4 to 6. No evident changes in afferent lymph were observed. The increase in efferent lymph was accompanied by an increase in lymph flow and total protein concentration. Up to 60% of the IgM and only a small amount of IgG1 in efferent lymph were newly synthesized in a lymph node undergoing an immune response.40
Evaluation of clinical, diagnostic features and therapeutic outcome of neurobrucellosis: a case series and review of literature
Published in International Journal of Neuroscience, 2022
Sudipta Patra, Vandana Kalwaje Eshwara, Aparna Ramakrishna Pai, Muralidhar Varma, Chiranjay Mukhopadhyay
Blood culture using the automated BacT/ALERT® 3D (bioMérieux, India) system and SAT using serum specimens were performed in all patients. Antibody titer ≥1:160 was considered as significant in serum specimens. CSF specimens were also subjected to culture, and tested for total cell count, protein, glucose, adenosine deaminase (ADA) and chloride. CSF abnormalities were considered as increased WBC count (>10 cells/mm3) with lymphocytic predominance, elevated protein levels (>45 mg/dL), and/or reduced glucose levels (<40 mg/dL). Isolated organisms were presumptively identified by Gram’s staining, oxidase test and Christensen’s urease test. Isolates were further confirmed by multiplex polymerase chain reaction (PCR) targeting the bcsp31 gene of Brucella for the simultaneous identification of genus Brucella (208 bp), Brucella abortus (498 bp), and Brucella melitensis (731 bp) [9].
Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2019
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
Triton X-100, cyclohexane, hexanol, ammonia (25–28 wt%) were purchased from Merck (Merck, Germany). 3-[2-(2aminoethylamino) ethylamino] propyl-trimethoxysilane (APTMS), Tetraethyl Orthosilicate (TEOS), Bovine Serum Albumin (BSA), Fe2O3, Fe3O4, 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich (Sigma-Aldrich, Merck, USA). C.I. Reactive Blue 21 was prepared by Santa Cruz (Santa Cruz, USA). Polyclonal antibody against Brucella abortus was purchased from Biorbyt, orb10564 (Biorbyt, USA). Brucella abortus 544, Brucella melitensis 16M, Escherichia coli O:157 ATCC4 3895, Salmonella typhimurium ATCC14028, Yersinia enterocolitica O:9 ATCC700823, Staphylococcus aureus ATCC49775 and Stenotrophomonas maltophilia ATCC13637 were purchased from Razi Vaccine and Serum Research Institute, Hesarak, Karaj, Iran, the water used was doubly distilled.
The antigenicity performance of divalent recombinant B. melitensis vaccines versus univalent ones
Published in Alexandria Journal of Medicine, 2019
Tooba Abbassi-Daloii, Soheil Yousefi, Mojtaba Tahmoorespur, Mohammad Hadi Sekhavati
These results are in agreement with some other reports that have shown efficient immune responses against Brucella associated with high levels of Th1 cytokines and IgG2a [7,13]. Luo et al. [20] and Luo et al. [23] showed divalent genetic vaccines elicit stronger cellular immune response and better protection against Brucella abortus than univalent vaccines. Also, Tadepalli et al. [1] studied immunogenicity efficacy of rOmp19, rP39, and rOmp19 + rP39 injections. They found rOmp19 + rP39 immunized mice induced significantly higher proliferative response with considerable cytokines expression, higher IgG2a antibody titer than univalent injected groups, as well. In another study, the simultaneous injection of HSP60 and chimeric BLS-OMP25 antigen showed better immunity than univalent injections of each recombinant protein [20]. However, Abbassi-Daloii et al. [24] found that the simultaneous injection of GroEL with OMP31 and OMP25 did not improve immune responses rather than individual injections of OMP31 and OMP25.