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Apiaceae Plants Growing in the East
Published in Mahendra Rai, Shandesh Bhattarai, Chistiane M. Feitosa, Ethnopharmacology of Wild Plants, 2021
Sherweit El-Ahmady, Nehal Ibrahim, Nermeen Farag, Sara Gabr
Foeniculum vulgare has been used as an ethnic remedy for the cure of numerous infectious disorders of bacterial, fungal, viral, and mycobacterial origin (Badgujar et al. 2014). The aqueous and methanolic extract of the dried seeds was shown to have a broad antibacterial activity and was individually tested against a number of pathogenic bacteria by the disc diffusion method. This was due to the presence of compounds such as linoleic acid, undecanal, 1, 3-benzenediol, oleic acid and 2, 4-undecadienal in the extract. It is reported that fennel seed extract also contains 5-hydroxy-furanocoumarin, which contributed to the antimicrobial property of this plant (Khan 2017). In another in vitro study, aqueous and alcoholic seed extracts of F. vulgare exhibited inhibitory effect against Alternaria alternata, Mucor rouxii, and Aspergillus flavus (Thakur et al. 2013). Twenty compounds were isolated from the hexane extract and tested against one sensitive and three MDR strains of Mycobacterium tuberculosis using the Alamar Blue microassay (Esquivel-Ferriño et al. 2012).
Protein amino-termini and how to identify them
Published in Expert Review of Proteomics, 2020
Annelies Bogaert, Kris Gevaert
The HYTANE method in essence modifies internal peptides with a hydrophobic moiety which traps these peptides on reverse-phase resins, thereby separating such peptides from N-terminal peptides [52]. Here, internal peptides generated from trypsin digestion of dimethylated proteins are modified with two hexadecanals through a Schiff base reduction reaction. In this way, internal peptides become highly hydrophobic, which explains the HYTANE acronym as hydrophobic tagging assisted N-termini enrichment. Weng and colleagues recently reported a HYTANE-like one-pot workflow for enriching N-terminal peptides termed HUNTER, which stands for high-efficiency undecanal-based N-termini enrichment [53]. HUNTER uses the paramagnetic SP3 beads-based workflow for proteome preparation which is steered by hydrophilic interactions [54]. Proteins are dimethylated and, following tryptic digestion, the α-amino groups of internal peptides are reacted with a hydrophobic undecanal group that allows their retention on reverse-phase resins. Their efficient workflow allows handling minute amounts of samples (e.g. 10,000 cells or just 2 µg of protein input material) as demonstrated by N-terminome analyses of sorted cells, mitochondria and liquid biopsies.
Salvia hispanica L. seeds extract alleviate encephalopathy in streptozotocin-induced diabetes in rats: role of oxidative stress, neurotransmitters, DNA and histological indices
Published in Biomarkers, 2022
Amal M. El-Feky, Marwa M. Elbatanony, Asmaa F. Aboul Naser, Eman A. Younis, Manal A. Hamed
In addition, the GC/MS analysis revealed the presence of twenty-two compounds from the unsaponifiable matter, representing 83.14%. The data showed the presence of eleven hydrocarbons (36.31%), four fatty alcohols (24.04%) and three aldehydes and ketones (19.11%) which were identified for the first time from S. hispanica L. petroleum ether seeds extract. Four previously reported sterols (3.68%) were also identified. Docosane (6.28%) was found to be the major identified hydrocarbon, while 1-tridecanol (12.66%) was the dominant fatty alcohol. Moreover, the most abundant aldehydes and ketones were undecanal (10.09%).