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Anticancer Properties of Silver Nanoparticles from Root Extract of Trigonella Foenum-Graecum
Published in Megh R. Goyal, Preeti Birwal, Santosh K. Mishra, Phytochemicals and Medicinal Plants in Food Design, 2022
Ramasamy Harikrishnan, Lourthu Samy S. Mary, Gunapathy Devi, Chellam Balasundaram
Mueller–Hinton Agar (MHA) was prepared and disinfected in a autoclave at 121 °C for 15 min (15 psi pressure) to investigate the antibacterial activity of biosynthesized TFAgNPs. Then sterile MHA (20 mL) was poured aseptically into each sterile petridish and accepted for solidification at RM under sterile conditions. After solidifying, petriplates were spread with MTCC cultures. The wells were bored in each plate by using a sterile borer. Twenty microliters of the sample (containing 5 mg/L) of the biosynthesized TFAgNPs together with standard antibiotic were laden with equal quantity in each well on the plates. Dimethyl sulfoxide (DMSO) and AgNO3 were used and loaded in respective wells for control. The petri plates were then kept at 37 °C and they measure the activity of clearly visible after 24 h. The ZI was determined and the sample of the biosynthesized TFAgNPs was noted as the highest antimicrobial activity.
Green Metal-Based Nanoparticles Synthesized Using Medicinal Plants and Plant Phytochemicals against Multidrug-Resistant Staphylococcus aureus
Published in Richard L. K. Glover, Daniel Nyanganyura, Rofhiwa Bridget Mulaudzi, Maluta Steven Mufamadi, Green Synthesis in Nanomedicine and Human Health, 2021
Abeer Ahmed Qaed Ahmed, Lin Xiao, Tracey Jill Morton McKay, Guang Yang
The green synthesis of GAgNPs was previously done using Cleome viscosa plant extract which were further characterized (Table 10.1) (Lakshmanan et al., 2018). The presence of biosynthesized GAgNPs (410–430 nm) was determined by UV-vis spectroscopy. FTIR spectrum tested the existence of various functional groups that act as capping agents for the GAgNPs. The GAgNPs morphology was analysed by SEM and the existence of silver was determined using elemental analysis. TEM revealed that the GAgNPs were well-dispersed and predominantly spherical in shape. Some GAgNPs exhibited irregular shapes between 5 and 30 nm. The antibacterial activity of GAgNPs was tested using the well diffusion method on Mueller–Hinton agar plates against S. aureus, Bacillus subtilis, Klebsiella pneumoniae and Escherichia coli. GAgNPs showed good antibacterial activity towards both gram-positive and gram-negative bacteria. The maximum inhibition zone for S. aureus was 17 ± 0.8 mm. The antibacterial activity of GAgNPs is concentration dependent. High levels of GAgNPs performed well in terms of inhibition activity against bacterial growth. Additionally, biosynthesized GAgNPs using Cleome viscosa plant extract proved effective against the growth of cancer cells in vitro, a possible indicator of an anticancer effect.
Potential of Piper Germplasm Against Pathogenic Bacteria: Tropical Bay Islands in India
Published in Megh R. Goyal, Durgesh Nandini Chauhan, Assessment of Medicinal Plants for Human Health, 2020
Chinthamani Jayavel, Ajit Arun Waman, Saravanan Kandasamy, Pooja Bohra
Antibacterial activity of the ethanolic extracts of Piper samples was determined using agar well diffusion method.16 For this, Mueller Hinton agar (HiMedia, Mumbai, India) plates were prepared and 50 µL of test culture was transferred aseptically to each plate and spread using a sterile L-rod followed by 30 min incubation. Using sterile cork borer (5.5 mm in diameter), four wells were made in each plate and 80 or 100 µL of prepared extract, as per the treatment, was inoculated in each well. Streptomycin (1 mg/mL) and chloramphenicol (1 mg/mL) were used as positive controls, whereas 80% ethanol was used as the negative control. Plates were then incubated at 37°C in an orbital shaking incubator (Remi, India). Each sample was maintained in triplicate to minimize the experimental error. Zone of inhibition in response to the challenging was measured after 24 h.
Alginate-based aerogels as wound dressings for efficient bacterial capture and enhanced antibacterial photodynamic therapy
Published in Drug Delivery, 2022
Ning Guo, Yu Xia, Weishen Zeng, Jia Chen, Quanxin Wu, Yaxin Shi, Guoying Li, Zhuoyi Huang, Guanhai Wang, Yun Liu
5,10,15,20-Tetra (4-aminophenyl) porphyrin (TPAPP) was purchased from Zhengzhou Anmusi Chemical Products Co., Ltd. (Zhengzhou, China). Sodium alginate (SA), 3-aminophenylboronic acid, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC·HCl), and CaCl2 were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Beijing, China). N-Hydroxysuccinimide (NHS) was purchased from Tianjin C&S Biochemical Technology Co., Ltd. (Shanghai, China). 1,3-Diphenylisobenzofuran (DPBF, 97%) was purchased from Acros Organics (Geel, Belgium). Luria–Bertani (LB) Broth powder was purchased from Beijing Solarbio Biotechnology Co., Ltd. (Beijing, China). Mueller Hinton agar medium was purchased from Guangdong Huankai Microbial Technology Co., Ltd. (Guangdong, China). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA, USA). SYTO 9 and propidium iodide (PI) were purchased from Sigma Co, Ltd. (St. Louis, MO, USA). All solvents and reagents were of analytical grade and used as received unless declared.
Chitosan-bovine serum albumin-Carbopol 940 nanogels for mupirocin dermal delivery: ex-vivo permeation and evaluation of cellular binding capacity via radiolabeling
Published in Pharmaceutical Development and Technology, 2021
Zeynep Ay Şenyiğit, Nesrin Coşkunmeriç, Emre Şefik Çağlar, İsmail Öztürk, Evren Atlıhan Gündoğdu, Panoraia I. Siafaka, Neslihan Üstündağ Okur
The inhibition zone diameters of nanogels against S. aureus were analyzed via disk diffusion test according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. The bacterial strain was grown on Mueller–Hinton agar (MHA, Merck, Germany) for one day at 35 °C. Following, the fresh colonies were suspended in physiological saline, while the inoculum suspensions were adjusted to 0.5 McFarland turbidity with h densitometer device (Den-1, Biosan, Latvia). In further, the MHA surface covered by the bacterial suspensions using sterile swabs, and followed by the air drying of the plates for 15 min. The sterile blank disks (6 mm in diameter, Oxoid, UK) were laid on the surface of the inoculated plates. Furthermore, 10 μL of each nanogel was inserted into the sterile disks, and the plates were incubated at 35 °C for 18 h. As a reference, an MPR disk (200 µg, Oxoid, UK) was used, and the quality control ranges analyzed in accordance with the EUCAST criteria. Every formulation was tested at least three times and mean inhibition zone diameters ± standard deviation (SD) values in mm were reported. The quality control of the MPR disk (200 µg/disk) against standard S. aureus strain ranged between 31 and 37 mm as stated by the EUCAST (EUCAST 2018).
Pseudomonas aeruginosa – Candida interplay: effect on in vitro antibiotic susceptibility of Pseudomonas aeruginosa when grown in the presence of Candida culture
Published in British Journal of Biomedical Science, 2021
R McIlroy, BC Millar, DW Nelson, A Murphy, JR Rao, DG Downey, JE Moore
The effect on antibiotic susceptibility when employing Mueller-Hinton agar supplemented with 10% [v/v] CCE from C. albicans, C. glabrata and C. parapsilosis is shown in Table 1. Statistically, there were significant differences in the eight PA isolates examined in mean zone sizes of the five antipseudomonal antibiotics, with the exception of C. glabrata and ciprofloxacin (p = 0.36) and meropenem (p = 0.07). Overall, when grown in the presence of CCEs, PA’s antibiotic susceptibility increased with all antibiotics, with the exception of C. albicans and C. parapsilosis with meropenem, where antibiotic susceptibility decreased. This is in contrast to the effect on PA susceptibility seen with the other β-lactam antibiotic examined, namely ceftazidime, where antibiotic susceptibility increased significantly in the presence of each of the three CCEs examined.