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Infant feeding practices and anaemia in 9-month-old infants
Published in Cut Adeya Adella, Stem Cell Oncology, 2018
D.P. Amri, T. Sembiring, P. Sianturi, T. Faranita, W. Pratita
The subjects of this study were 9-month-old infants brought to puskesmas or posyandu, as mentioned above. The subjects who met the inclusion criteria were recruited using the consecutive sampling method. Parents were interviewed to obtain a socio-economic characteristic of the family along with infant feeding practices (e.g. breastfeeding exclusively, the age when complementary feeding started, when the first complementary food was given, the animal protein content, the preparation of complementary food and the frequency of giving one type of complementary food). The subjects’ body weight and length were measured, and blood haemoglobin levels measured using a HemoCue® Hb 201+System device. This research received ethical approval from the Ethical Research Committee, Faculty of Medicine, University of Sumatera Utara, and has also been approved by the local public health office, puskesmas and posyandu, in which this research took place.
Effect of Diets Varying in Iron and Saturated Fat on the Gut Microbiota and Intestinal Inflammation: A Crossover Feeding Study among Older Females with Obesity
Published in Nutrition and Cancer, 2023
Patricia G. Wolf, Beatriz Penalver Bernabe, Manoela Lima Oliveira, Alyshia Hamm, Andrew McLeod, Sarah Olender, Karla Castellanos, Brett R. Loman, H. Rex Gaskins, Marian Fitzgibbon, Lisa Tussing-Humphreys
At baseline, hemoglobin was obtained using a point of care analyzer (HemoCue, Brea, CA). Hemoglobin < 11.0 g/dL was indicative of anemia. A fasting venous blood sample was obtained at the baseline, each diet baseline, and within 48 hours of completing a given diet sequence. Subjects were asked to fast for a minimum of 12 hours, and refrain from exercising with high intensity for 24 hours, or taking NSAIDS, Cox-2 inhibitors, steroids, endocrine treatments, vitamins, or supplements for a minimum of 12 hours prior to the blood draw. They were also asked to avoid taking statin medications the morning of the appointment. Blood was processed for serum and plasma following standard procedures and shipped to a local commercial lab (Quest Diagnostics, Wood Dale, IL) or stored at −80 °C until analysis. Serum iron and ferritin were measured by immunoassay and spectrophotometry, respectively; high sensitivity C-reactive protein (hs-CRP) by immunoturbidimetry; glucose by spectrophotometry; and insulin by immunoassay at a local certified commercial lab (Quest Diagnostics, Wood Dale, IL). Serum hepcidin was measured using a commercially available immunoassay kit (Intrinsic LifeSciences La Jolla, CA) with a lower limit of detection of 2.5 ng/ml. Samples for hepcidin were run in duplicate by a laboratory technician blinded to diet sequence.
Spaceflight medical countermeasures: a strategic approach for mitigating effects from solar particle events
Published in International Journal of Radiation Biology, 2021
In the event of a SPE, it will be important to have a device capable of measuring white blood cell count (WBC) with differential in order to determine if a crew member needs to take a medical countermeasure for possible neutropenia. Currently, HemoCue® is the only point-of-care device commercially available that is capable of providing a WBC count with differential. It requires a finger stick and provides results in minutes. The proposed G-CSF, Peg-G-CSF and GM-CSF medical countermeasures are recommended to be delivered if the absolute neutrophil count (ANC) drops below 1000/mm3 with regular monitoring of ANC recommended and additional doses of G-CSF or GM-CSF administered as prescribed (Amgen 2015; FDA 2018). Several new technologies are currently in development that may provide complete blood count (CBC) in miniaturized, rapid reporting devices. NASA is currently monitoring the development of these devices with the goal of including one on long duration missions.
Spot Sample Urine Specific Gravity Does Not Accurately Represent Small Decreases in Plasma Volume in Resting Healthy Males
Published in Journal of the American College of Nutrition, 2018
Matthew A. Tucker, Cory L. Butts, Alf Z. Satterfield, Ashley Six, Evan C. Johnson, Matthew S. Ganio
Blood samples were collected via an intravenous catheter (SurFlash, Terumo Corporation, Tokyo, Japan) placed in a superficial forearm vein while subjects were in a reclined position in a phlebotomy chair. Whole blood was drawn into a 6-ml Vacutainer collection tube with EDTA additive for analysis of hemoglobin (Hb), hematocrit (Hct), and carboxyhemoglobin concentration (HbCO%). A 4-ml clot activator tube was used for analysis of serum osmolality (Sosm), sodium, potassium, chloride, and protein (Spro). Osmolality was measured in duplicate fresh samples using freezing point depression osmometry. Hemoglobin was measured in triplicate 10-µl samples using a HemoCueHb 201+ analyzer (HemoCue AB, Angelholm, Sweden). Hematocrit was analyzed in triplicate 35-µl samples drawn into microcapillary tubes, spun down in a microcentrifuge for 3 minutes at 12,000 rpm (model C-MH30, UNICO, Dayton, NJ), and values measured on a Micro-Capillary Reader (International Equipment Company, Needham Heights, MA). Aliquots of serum were stored at −80°C before being thawed and analyzed for sodium, potassium, and chloride in duplicate using a Medica Easy Electrolytes analyzer (Medica Corporation, Bedford, MA) and Spro using a handheld refractometer (Master-SUR/NM).