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Histological Study in Orthopaedic Animal Research
Published in Yuehuei H. An, Richard J. Friedman, Animal Models in Orthopaedic Research, 2020
Helen E. Gruber, Audrey A. Stasky
Small undecalcified bone specimens embedded in glycol or methyl methacrylate can be cut using an appropriate motor-driven microtome and tungsten carbide knives (for methyl methacrylate or glycol methacrylate) or large glass knives (for glycol methacrylate).
Autoradiography
Published in Howard J. Glenn, Lelio G. Colombetti, Biologic Applications of Radiotracers, 2019
Sven Ullberg, Bengt Larsson, Hans Tjälve
Soluble compounds have so far not been successfully localized. In the preparation of ultrathin tissue sections, customary techniques are therefore almost always used. They involve, for example, fixation with glutaraldehyde and osmium tetroxide, dehydration, and embedding in resins. The sectioning is done on a liquid surface with a glass knife. For emulsion application, dipping in diluted fine-grained liquid nuclear emulsion seems to dominate. Before being dipped, the sections need to be stretched on a flat support such as a glass slide. After exposure and development, they are transferred to grids for examination in the electron microscope.
Calanus oil attenuates isoproterenol-induced cardiac hypertrophy by regulating myocardial remodeling and oxidative stress
Published in Ultrastructural Pathology, 2023
Shrook Y. Abdellatif, Nagui H. Fares, Samar H. Elsharkawy, Yomna I. Mahmoud
After dissection, the left ventricles were cut into small pieces and then immersed in fresh ice cold 2.5% glutaraldehyde in 0.l M phosphate buffer at pH 7.2 for a few hours. After that, the samples were rinsed three times in the buffer and post-fixed for 2 hours in 1% phosphate-buffered OsO4, dehydrated through ascending graded series of alcohol, embedded in resin, and left to polymerize overnight at 70°C. Semithin sections (~0.5 µm) were cut with glass knives, mounted on glass slides, and stained with 1% toluidine blue O and examined with a light microscope at 400X magnification. Areas of interest were selected and blocks were trimmed accordingly. Ultrathin sections (0.1 μm) were collected on grids and double-stained in uranyl acetate and lead citrate. Then sections were visualized and photographed using Jeol 100S transmission electron microscope (Tecnai, Hitachi, Tokyo, Japan) in the Regional Mycology and Biotechnology Center at Al-Azhar University.in the Regional Mycology and Biotechnology Center at Al-Azhar University.
Ultrastructural analysis of cadmium-induced toxicity and its alleviation by antioxidant quercetin in caprine testicular germ cells in vitro
Published in Ultrastructural Pathology, 2022
Harish Panchal, Som Nath Sachdeva, Jitender Kumar Bhardwaj
After the respective treatment durations, the testicular tissues from both untreated and treated groups were fixed in glutaraldehyde (2.5%) in phosphate buffered saline (0.2 M) for 24 hours at 4°C. Testicular tissue was again trimmed to the appropriate size (˜1 mm3) in buffer and fixed in osmium tetroxide (1.3%) at 4°C for 2 hours. Post-fixation, the tissue was dehydrated through graded series of acetone, cleared in epoxy propane, and embedded in epoxy-resin blocks. The blocks were initially sectioned at 1 μm thickness before being examined under a phase contrast microscope to select the required tissue area. Post-selection, the tissue was sectioned at a thickness of 60–90 nm with glass knives and mounted on mesh grids. Finally, the tissue sections were stained with uranyl acetate and lead citrate before being examined (two samples in each group) under Morgagni 268 D TEM from FEI, the Netherlands, installed at the All India Institute of Medical Sciences (AIIMS), New Delhi.25,26
Transmission electron microscopic analysis of glyphosate induced cytotoxicity and its attenuation by N-acetyl-L-cysteine in caprine testicular germ cells in vitro
Published in Ultrastructural Pathology, 2021
Jitender Kumar Bhardwaj, Vijay Kumar, Harish Panchal, Som Nath Sachdeva
After GLY treatment along with NAC supplementation, the testicular tissues were fixed in 2.5% glutaraldehyde in 0.2 M phosphate buffer saline (PBS) (pH 7.2–7.4) at 4°C for 24 h. After fixation, tissues were finally trimmed to appropriate sizes (˜1 mm3), post-fixed in 1.3% osmium tetraoxide at 4°C for 2 h followed by dehydration in graded acetone series. Tissues were cleared in epoxy propane and its embedding was done in epoxy-resin blocks. For selection of the required area, initially tissues were sectioned at 1 µm thickness and observed under phase-contrast microscope. After selection of the required area, tissue sections of 60–90 nm thickness were obtained using glass knives and mounted on mesh grids. The sections were stained with uranyl acetate and lead citrate followed by their examination (two samples in each group) under Morgagni 268 D TEM from FEI, Netherlands, installed at the All India Institute of Medical Sciences (AIIMS), New Delhi.8,31