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Fluorescent Technology in the Assessment of Metabolic Disorders in Diabetes
Published in Andrey V. Dunaev, Valery V. Tuchin, Biomedical Photonics for Diabetes Research, 2023
Elena V. Zharkikh, Viktor V. Dremin, Andrey V. Dunaev
A large amount of diagnostic information is provided not only by fluorescence spectra and two-dimensional image registration, but also by time-resolved measurements of fluorescence lifetime. Fluorescence lifetime imaging (FLIM) provides additional diagnostic information by measuring tissue autofluorescence lifetime, helping to separate fluorophores with close overlapping absorption and emission spectra. One of the significant applications of FLIM is the in vivo assessment of the metabolic status of tissues based on the measurement of the fluorescence lifetime of free and protein-bound NADH [9,10].
Therapeutic Strategies and Future Research
Published in Mark A. Mentzer, Mild Traumatic Brain Injury, 2020
Optical imaging techniques include fluorescence microscopy, Raman imaging, interference imaging, optical coherence tomography, total internal reflection imaging, multi-photon microscopy, confocal microscopy, and other developing tools, including fluorescence imaging. Fluorescence optical imaging systems include spatial filtering confocal microscopy, spatially resolved localized spectroscopy, polarization and time-resolved fluorescence lifetime imaging (FLIM), and fluorescence resonance energy transfer (FRET). Applications include whole body imaging, drug distribution, protein engineering, and identification of structural changes in cells, organelles, and tissues (Prasad, 2003).
Application of Nonlinear Microscopy in Life Sciences
Published in Lingyan Shi, Robert R. Alfano, Deep Imaging in Tissue and Biomedical Materials, 2017
Zdenek Svindrych, Ammasi Periasamy
By far the most common multiphoton instrumentation employed for biological imaging is single spot laser scanning configuration based on a confocal laser scanning microscope. It is readily available, and further advances in femtosecond laser sources will make it even more common imaging modality. It is being used extensively in many areas of biological imaging, ranging from subcellular studies to whole organ or whole body imaging in neuroscience, developmental biology and cancer research, to name just a few. While adding fluorescence lifetime measurement capabilities is straightforward and not uncommon, there are only few studies using lifetime-based sensors or FLIM-FRET in the context of deep tissue imaging. On the other hand, FLIM-based quantitative studies of endogenous fluorophores (NADH, FAD) are quickly gaining popularity.
A review of recent advances in nanodiamond-mediated drug delivery in cancer
Published in Expert Opinion on Drug Delivery, 2021
Elena Perevedentseva, Yu-Chung Lin, Chia-Liang Cheng
Other techniques, fluorescence imaging with two-photon excitation with low out-of-focus excitation and increasing spatial resolution, safer for biosample when infrared excitation is used [39–41]. Fluorescence lifetime imaging microscopy (FLIM) allows studies of the interaction between NDs and the sample via simultaneous estimation of the sample conditions by analyzing the lifetimes of endogenous fluorophores and their spatial distribution [42]. Optical and spectroscopic properties of ND, as well as ND physical properties in general, make ND very attractive for theranostic applications, allowing drug delivery, tracing, and detection in the bio/medical applications [1–5,7,8].