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Behind the scenes of the forensic lab
Published in Rachel E. Lovell, Jennifer Langhinrichsen-Rohling, Sexual Assault Kits and Reforming the Response to Rape, 2023
The following items of evidence were collected in Case 1's SAK:Vaginal swabAnal/perianal swabBuccal swab (known DNA swab)Face swab of cheekSwab of breastsSwab of mouth
Research with Human Subjects:
Published in Lynne M. Bianchi, Research during Medical Residency, 2022
Lynne M. Bianchi, Joyce Babyak, Robert Maholic
Examples of projects that may fall under the expedited review category are those that collect voice recordings for research purposes, non-invasive collection of biological specimens for research purposes (e.g., nail clippings, mucosal cells via buccal swab), or use of approved medical devices as intended.
Sexual Abuse, Sex Trafficking, and Rape
Published in S Paige Hertweck, Maggie L Dwiggins, Clinical Protocols in Pediatric and Adolescent Gynecology, 2022
Jacqueline Sugarman, S. Paige Hertweck
Specimens collected and placed in the kit include Buccal swab (to identify the patient's DNA)Underwear that the patient was wearing at the time of the assaultSwabs of any dried or moist secretions observed on the patient's bodySwabs of the oral cavity, vagina, and anorectum
Exploration of the interplay between spatially distinct microbial habitats through comparative analysis
Published in Journal of Oral Microbiology, 2023
Hyunji Kim, Jin-Sil Hong, Pil-Young Yun, Kyung-Gyun Hwang, Keun-Suh Kim, Hyo-Jung Lee, Kyoung Un Park
This study utilized genomic DNA (gDNA) specimens obtained from the Periodontal Human Specimen Storage Registry at Seoul National University Bundang Hospital. The specimens were collected between 2015 and 2019, and their use for secondary research purposes was approved by the provider within the designated storage period. The registry specimens consisted of five spatially distinct specimens: saliva, buccal swabs, plaque from the oral cavity, stool, and blood. The collection followed a standardized protocol used in the HMP [23,27]. An experienced periodontist recorded the collection and group classification of oral microbial specimens. The collection of oral cavity specimens followed a specific order: buccal swabs, saliva, and plaque. Blood samples were collected on the same day as saliva collection. Patients were instructed to fast for 8 hours and abstain from oral hygiene for at least 2 hours before the collection of oral specimens. The buccal swab samples were collected from the inner buccal mucosa of the right and left cheek using cotton swabs provided with the buccal swab kit. Saliva was collected for 20 minutes without stimulation. All periodontal probing depths were pre-recorded, and subgingival plaque was taken from the two deepest pockets during sampling. The gDNA was isolated from each specimen using commercial kits following the manufacturer’s instructions (Supp. Text 1). Extracted DNA was prepared for sequencing according to the protocol of the HMP consortium [28].
Insights into Punic genetic signatures in the southern necropolis of Tharros (Sardinia)
Published in Annals of Human Biology, 2021
Stefania Sarno, Elisabetta Cilli, Patrizia Serventi, Sara De Fanti, Andrea Corona, Francesco Fontani, Mirko Traversari, Gianmarco Ferri, Anna Chiara Fariselli, Donata Luiselli
The sampling was carried out with stringent in-situ procedures to minimise the risk of contamination and increase DNA preservation (Pruvost et al. 2007; Bollongino et al. 2008; Fortea et al. 2008; Llamas et al. 2017). In particular: (i) all ancient specimens were collected using disposable lab coat, sterile gloves, face mask, hair net, over-shoes and all the equipment used was decontaminated with 5% NaClO and Ethanol before and after each sampling; (ii) the freshly excavated specimens were stored in specific plastic bags (annotated with sample description, location, number of burial and stratigraphic unit) and delivered to the laboratory of analysis; (iii) buccal swab samples from all the personnel involved in the study (archaeologists, anthropologists and laboratory researchers) were also collected to monitor for potential sources of contamination.
Androgen receptor gene microsatellite polymorphism is associated with muscle mass and strength in bodybuilders and power athlete status
Published in Annals of Human Biology, 2021
João Paulo L. F. Guilherme, Yulia V. Shikhova, Rimma R. Dondukovskaya, Alexandra A. Topanova, Ekaterina A. Semenova, Irina V. Astratenkova, Ildus I. Ahmetov
Molecular genetic analysis was performed with DNA samples obtained from epithelial mouth cells by alkaline extraction or using a DNK-sorb-A sorbent kit according to the manufacturer’s instruction (Central Research Institute of Epidemiology, Moscow, Russia), depending on the method of sample collection (buccal swab or scrape). Genotyping for the AR gene (CAG)n polymorphism was performed by polymerase chain reaction (PCR) on a Tercyk thermal cycler (DNA Technology, Moscow, Russia). The DNA was amplified using two primers (5′-TCCAGAATCTGTTCCAGAGCGTGC-3′ and 5′-GCTGTGAAGGTTGCTGTTCCTCAT-3′) (Litech, Moscow, Russia). The length of amplified fragments (≈ 260–280 base pairs) varied only by the number of CAG repeats. For accurate assessment of fragment length, the DNA fragments were run on a polyacrylamide gel (6%). The lengths of unknown PCR products were calculated using DNA λ-markers (Sibenzyme, Novosibirsk, Russia). All genotyping analyses were conducted in duplicates blind to subject identity.