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Nanophyetus
Published in Dongyou Liu, Handbook of Foodborne Diseases, 2018
Neorickettsia helmintheoca is a coccoid to coccobacillary bacterium (family Rickettsiaceae) of 0.3 μm in size, appears purple (i.e., gram negative) in Giemsa stain, and occurs in all stages of the trematode. The complete genome sequence of N. helminthoeca Oregon reveals a single, small circular chromosome of 884,232 bp encoding 774 potential proteins. While N. helminthoeca does not seem to produce lipopolysaccharides and most amino acids, it is capable of synthesizing vitamins, cofactors, nucleotides and bacterioferritin. In addition, by encoding nearly all enzymes required for peptidoglycan biosynthesis, N. helminthoeca has certain structural hardiness and inflammatory potential [17,18].
Rickettsia spp.
Published in Peter M. Lydyard, Michael F. Cole, John Holton, William L. Irving, Nino Porakishvili, Pradhib Venkatesan, Katherine N. Ward, Case Studies in Infectious Disease, 2010
Peter M. Lydyard, Michael F. Cole, John Holton, William L. Irving, Nino Porakishvili, Pradhib Venkatesan, Katherine N. Ward
The patient has a rickettsial infection. The taxonomy and nomenclature of the Rickettsiaceae have undergone a major revision based upon 16S rRNA sequences. Currently the Rickettsiales contain the Rickettsia, Orientia, Ehrlichia, Anaplasma, Wolbachia, and Neorickettsia. Coxiella burnetti is now included in the γ-proteobacteria along with Legionella and Francisella. Frequently Rickettsia are given species names relating to their original geographic location. There are many species of Rickettsia found in nature, only some of which have been linked to illness (Table 1) and present clinically as spotted fevers or typhus (see later).
A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America
Published in Pathogens and Global Health, 2020
Gabriel L. Cicuttin, María N. De Salvo, Paula Díaz Pérez, Darío Silva, María L. Félix, José M. Venzal, Santiago Nava
PCR-products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA) and sequenced with a 3500 Genetic Analyzer sequencer (Applied Biosystems, Foster City, USA). Sequences were edited using BioEdit Sequence Alignment Editor [34] with manual edition whenever it was necessary, aligned with the program Clustal W [35] and compared with those sequences of Ehrlichia and Anaplasma deposited in GenBank by using BLAST (www.ncbi.nlm.nih.gov/blast). Phylogenetic analyses were performed with Maximum-likelihood (ML) and best-fitting substitution models were determined with the Akaike Information Criterion using the ML model test implemented in MEGA 5.0 [36]. Support for the topologies was tested by bootstrapping over 1000 replications and gaps were excluded from the comparisons. Sequences of Anaplasma marginale (dsb) and Neorickettsia risticii (groESL) were included as outgroup.