Gordonia

Cytochrome P450 Enzymes for the Synthesis of Novel and Known Drugs and Drug Metabolites

Published in Peter Grunwald, Pharmaceutical Biocatalysis, 2019

Sanjana Haque, Yuqing Gong, Sunitha Kodidela, Mohammad A. Rahman, Sabina Ranjit, Santosh Kumar

CYP153A has been identified as a fatty acid ω-hydroxylase with a broad substrate range (Honda Malca et al., 2012). CYP153As can be obtained from variety of bacteria including Marinobacter aquaeolei, Alcanivorax borkumensis, and Gordonia alkanivorans (Jung et al., 2016). Recently, the CYP153A enzyme from M. aquaeolei was engineered to a double mutant G307A/S233G based on semi-rational protein design to improve the activity of terminal hydroxylation of medium- and long-chain fatty acids. The most active mutant showed a 3.7-fold improvement over the wild-type activity. Additionally, the heme domain of CYP153A mutant was constructed with the reductase domain of CYPBM3 to improve the catalytic activity for its future industrial applications (Notonier et al., 2016b). Moreover, a crystal structure of CYP153A from M. aquaeolei revealed a crucial target for engineering CYP153A. CYP153A residues at the extended F-helix and Ω-loop were targeted using site-directed mutagenesis. Importantly, mutant P135A showed an enhanced activity on hydroxylation of hexadecanoic acid with 20% more conversion than the wild-type enzyme (Hoffmann et al., 2016).

Published in Ronald M. Atlas, James W. Snyder, Handbook Of Media for Clinical Microbiology, 2006

Ronald M. Atlas, James W. Snyder

Use: For the cultivation of Corynebacterium spp., Streptomyces flocculus, Mycobacterium spp., Nocardia spp., Rhodococcus spp., Dermatophilus congo-lensis, and Gordonia amicalis.

Drip irrigation biofouling with treated wastewater: bacterial selection revealed by high-throughput sequencing

Published in Biofouling, 2019

Kévin Lequette, Nassim Ait-Mouheb, Nathalie Wéry

Dripper–pipe group separation was significantly dominated by Aquabacterium (OTU1). Dripper–TWW group separation was mainly driven by Terrimonas (OTU2), the OTU10 affiliated to the order Burkholderiales (MWH-UniP1 aquatic group) and the OTU13 affiliated to the class Actinobacteria (PeM15_ge) with a cumulative contribution of 11%. Cyanobacteria such as Synechococcus (OUT24) also contributed to the dripper–TWW divergence (Supplemental material Table S4). Pipe–TWW group separation was mainly driven by a member of the order Burkholderiales (MWH-UniP1 aquatic group, OTU10), the genus Aeromicrobium (OTU16) and the genus Brevundimonas (OTU20) with a cumulative contribution of 9%. Others such as Thiobacillus (OTU14), Chryseobacterium (OTU22), PeM15_ge (OTU13), Gordonia (OTU19) and Dietzia (OTU32) (Table 4, Supplemental material Table S3) also significantly contributed to the Pipe–TWW divergence.

Tsukamurella pulmonis central venous catheter infection mimicking proteinase 3-antineutrophil cytoplasmic antibody (PR3-ANCA)-associated vasculitis

Published in Immunological Medicine, 2021

Kiyoaki Ochi, Tomoyuki Mukai, Shigeru Ota, Chihiro Hiraiwa, Masahiko Ikeda, Airi Ikeda, Takashi Oda, Youichiro Yamamoto, Toru Ueki

Tsukamurella species are aerobic gram-positive rods that are found in a broad range of environments such as soil, water, and sludge [3,4]. Tsukamurella species are categorized to the order Actinomycetales and have many features similar to other Actinomycetales, such as Nocardia, Rhodococcus, Gordonia, and rapidly growing Mycobacterium bacteria [3]. Hence, Tsukamurella species can be misidentified as one of these genera using standard microbiological tests [3]. However, the identification of Tsukamurella can be achieved by 16S ribosomal RNA gene sequencing [4,5]. Clinical features of Tsukamurella infection include catheter-related bloodstream infections, skin and soft tissue infections, respiratory tract infections, conjunctivitis, and brain abscesses [3,4,6]. In the present case, Tsukamurella infection developed as a catheter-related bloodstream infection. Although most reported cases of Tsukamurella bacteremia were limited to immunocompromised patients [3,6–8], our patient was a non-immunocompromised individual. This suggests that Tsukamurella infection can occur not only in immunocompromised individuals but also in immunocompetent individuals on a specific occasion, such as intravenous catheter insertion. Since Tsukamurella species have been misdiagnosed as other Actinomycetales in the absence of correct molecular diagnostic methods [4,5], the importance of Tsukamurella species in disease presentation and pathogenesis may have been underestimated in clinical settings. Therefore, appropriate diagnosis and accumulation of clinical data are required to develop proper therapeutic strategies for Tsukamurella infection.

Bacteria Associated with Granulomatous Lobular Mastitis and the Potential for Personalized Therapy

Published in Journal of Investigative Surgery, 2022

Xin-Qian Li, Hong-Li Wu, Jing-Ping Yuan, Tian-gang Liu, Sheng-Rong Sun, Chuang Chen

Other bacteria associated with GLM have been reported. For example, Wang et al. [9] identified the five most abundant pathogenic genera in GLM as Pseudomonas, Brevundimonas, Stenotrophomonas, Acinetobacter and Aspergillus. Actinomyces has also been identified in GLM [41]. Fujii et al. [29] detected common sequences of Nocardia, Mycobacterium, Rhodococcus equi, Gordonia and Dietzia in two cases. In addition, mixed strains of Corynebacterium, Propionibacterium acnes, group B Streptococcus, and Candida were isolated in four cases [30].