Cyanophages

Cyanophages

RNA

Published in Paul Pumpens, Single-Stranded RNA Phages, 2020

The MS2 RNA was used by the generation of a novel method for the 3′-end-labeling of various types of RNA molecules with digoxigenin, or biotin-modified nucleotides, using terminal deoxynucleotidyl transferase (Rosemeyer et al. 1995). With the MS2 RNA as a prototype, Syrchin and Mendzhul (2002) applied the terminal transferase for the generation of the 32P-labeled hybridization probes by their studies on a cyanophage.

Virus-like particle-based nanocarriers as an emerging platform for drug delivery

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Journal Information

Published in Journal of Drug Targeting, 2023

Bingchuan Yuan, Yang Liu, Meilin Lv, Yilei Sui, Shenghua Hou, Tinghui Yang, Zakia Belhadj, Yulong Zhou, Naidan Chang, Yachao Ren, Changhao Sun

Some traditional methods, including ELISA, BCA or the Bradford method, and ultraviolet spectrophotometry, could be applied to quantify VLPs. Some new quantification methods have also been reported, for example, Goldin et al. used the polony method to quantify T4-like and T7-like cyanophages [149]. In addition, flow cytometry was applied to quantify human norovirus VLPs binding to commensal bacteria [150]. Its immunogenicity was detected using in vitro tests (e.g. haemagglutination inhibition tests) and in vivo immune tests (e.g. immunity and infection).

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Virus-like particle-based nanocarriers as an emerging platform for drug delivery