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Extracorporeal Purging of Bone Marrow Grafts by Dye-Sensitized Photoirradiation
Published in Adrian P. Gee, BONE MARROW PROCESSING and PURGING, 2020
From a purely technical point of view, we would prefer to use fetal bovine serum, even for the processing of clinical marrow grafts. During the past 11 years, we must have used in excess of a dozen different lots of fetal bovine serum for marrow purging experiments, yet we have identified only one lot that caused irreproducible results because its photoprotective power declined rapidly upon storage. With human serum, the experience is much more limited and no information is available about the frequency of “unstable” lots. On the other hand, there are legitimate concerns about the potential transmission of bovine viruses, and, last but not least, regulatory approval is obtained more easily for a human than for a bovine blood product.
Genetics and Molecular Biology of Adipose Cell Characteristics
Published in Claude Bouchard, The Genetics of Obesity, 2020
Gérard P. Ailhaud, Paul A. Grimaldi, Raymond L. Négrel
The observation that dormant preadipocytes are present in old animals as well as in elderly people has led to the identification of a panoply of adipogenic signals which are required in vitro for the terminal differentiation of preadipocytes. The role played by various hormones and factors in differentiating cells should not be confused with the role of the same hormones and factors in differentiated cells. In addition, because of uncertainty about the developmental stage of precursor cells from various sources at the time of inoculation, the characterization of cellular factors (adipogenic and antiadipogenic factors) has been and remains controversial. To add further complication, the (fetal) bovine serum used in most experiments contains positive and negative effectors, the proportions of which cannot be controlled. This makes comparisons difficult and has led investigators to use serum-free, chemically defined culture media.
The use of Spheroids in the Study of Invasion
Published in Rolf Bjerkvig, Spheroid Culture in Cancer Research, 2017
Marc Bracke, Hans Romijn, Vakaet Luc, Barbara Vyncke, Marc de Mets, Marc Mareel
Fetal bovine serum is a complex mixture of, amongst other components, growth factors, adhesion and spreading factors, hormones, and enzyme inhibitors. Serum (5 to 10%, v/v) is necessary for the survival of some cell types in confronting culture, while other cell types are less serum dependent. MO4 and MDCK cells can be confronted successfully with PHF in serum-free media, and these experiments have shown that the assay still discriminates between invasive and noninvasive cells when serum is omitted during confronting culture.32
A HER2-targeted antibody-novel DNA topoisomerase I inhibitor conjugate induces durable adaptive antitumor immunity by activating dendritic cells
Published in mAbs, 2023
Xiaoding Tan, Peng Fang, Kaiying Li, Meng You, Yuxia Cao, Hui Xu, Xiaohong Zhu, Lu Wang, Xin Wei, Haiying Wen, Wendi Li, Lei Shi, Xiaowei Sun, Dongan Yu, Huikai Zhu, Zhenzhen Wang, Datao Liu, Hui Shen, Wei Zhou, Maomao An
The following cells lines were purchased from the American Type Culture Collection: human breast cancer cell lines BT474 and SK-BR-3, human gastric cancer cell line NCI-N87, human pancreatic cancer cell line BxPC-3, mouse colon cancer cell line CT26.WT, and mouse breast cancer cell line EMT6. Cells were cultured in appropriate media containing 10% fetal bovine serum (FBS). Human breast cancer cell line MDA-MB-468 was purchased from the National Collection of Authenticated Cell Cultures of Chinese Academy of Sciences and cultured in medium containing 10% FBS. CT26.WT-HER2, a cell line stably transfected with the full-length human HER2 gene, was purchased from BYinno Biotechnology Co., Ltd. and cultured in Dulbecco’s modified eagle medium containing 10% FBS and 20 μg/mL puromycin (Gibco, Cat# 15070–063). The dendritic cells were purchased from Milestone (Shanghai) Biological Science & Technology Co. Ltd. and the informed consent for the scientific research in this study was obtained from the dendritic cells’ donors.
Serum Levels Evaluation of TMSB10 and Endocan in Breast Cancer Patients and In-Vitro Study of Chrysin Effect on These Markers in MCF-7 cell line
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Nada E. Hammouda, Yara A. Samra, Ziad Emarah, Amal M. El-Gayar
Doxorubicin (Dox 50 mg/25 mL) intravenous vial was purchased from Ebewe Pharma (Unterach, am Attersee, Austria). The tetrazolium dye assay was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT). It was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). DMSO (dimethyl sulfoxide) was obtained from Sigma–Aldrich (St. Louis, MO, USA). Trypsin-Versene (EDTA) Mix (1×), PBS-1× (Phosphate buffered Saline), penicillin–streptomycin stock antibiotic and DMEM (Dulbecco’s Modified Eagle’s Medium) were obtained from Lonza Verviers SPRL, Belgium. Sterile-filtered fetal bovine serum (FBS) was obtained from Life Science Group, UK. All other chemicals were of high analytical grade. Chrysin was purchased from Sigma Aldrich (Darmstadt, Germany) with CAS number c80105-25 G. It was weighted and dissolved in DMSO to prepare a concentration of 6.25, 12.5, 25, and 50 µg/mL.
Culturing human pluripotent stem cells for regenerative medicine
Published in Expert Opinion on Biological Therapy, 2023
Hiroki Ozawa, Takuya Matsumoto, Masato Nakagawa
ISO20399:2022 sets out the requirements and recommendations for both suppliers and users of AMs to ensure the safety and performance of manufactured cell products [43]. Moreover, there are variations in the emphasis placed on relevant guidelines and regulations among different countries [49]. For example, USP1043 guides the development of appropriate qualification programs for AMs used in cell, gene, and tissue-engineered products in the US [46]. In contrast, the EU EP5.2.12 focuses on materials extracted from biological sources and/or produced by recombinant DNA technology and addresses risk assessment, manufacturing, and quality control [50]. Japan has established the Standards for Biological Ingredients (SBIs) to ensure the safety of cell transplants and other products. These guidelines regulate using raw materials, animals, plants, and microorganisms and their products in pharmaceuticals, cosmetics, and foods. For example, fetal bovine serum (FBS) used in cell culture must follow these guidelines. Medical applications of hPSCs must also comply with these guidelines to ensure product quality and safety [51].