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ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF Delonix elata Linn.
Published in V. R. Mohan, A. Doss, P. S. Tresina, Ethnomedicinal Plants with Therapeutic Properties, 2019
It was observed that during the search of analgesic effect of selected extracts of the plant by hot-plate method, pentazocine showed significant analgesic effect at 30, 60, 120, and 180 min. Peak effect was observed at 120 min. Normal control (group I) did not have any significant change in basal reaction time. Methanol extract showed highly significant effect (p < 0.01) at 30, 60, 120, and 180 min as compared with control group. The aqueous extract 200 mg/kg showed a significant activity (p < 0.05) at 30 min and highly significant activity (p < 0.01) at 60, 120, and 180 min (Table 12.3). The hot-plate method is considered to be selective for the drugs acting centrally. The hot-plate test measures the complex response to a noninflammatory, acute nociceptive input and is one of the models normally used for studying central nociceptive activity (Sabina et al., 2009). It is an established fact that any agent that causes a prolongation of the hot-plate latency using this test must be acting centrally (Ibironke and Ajiboye, 2007). Therefore, the crude extracts of the plant must have a central activity. Again, narcotic analgesics inhibit both peripheral and central mechanism of pain, while NSAIDs inhibit only peripheral pain (Elisabetsky et al., 1995; Pal et al., 1999).
Cerebrospinal fluid neurotransmitter levels and central nervous system depression in a rat drug overdose model
Published in Toxicology Mechanisms and Methods, 2020
Hiroshi Tsutsumi, Kosei Yonemitsu, Ako Sasao, Yuki Ohtsu, Shota Furukawa, Yoko Nishitani
The hot plate test was performed in accordance with previously reported methods (Yamamoto et al. 2009). Rats were placed into an open-ended cylindrical space with a floor consisting of a 52.5 °C metallic hot plate (Model LE7406, Panlab, Comella, Spain). The response latency of either a hind-paw lick or a jump was recorded in seconds (s). In the absence of a response, animals were removed from the hot plate at 60 s (cut-off time), and a latency of 60 s was assigned as the response time. Tests were performed before and 2 h after drug administration. The peak serum concentration of phenobarbital has been reported to occur 2 h after oral administration of a dose of 100 mg/kg in rats (Kojima et al. 1971). Prolongation of the response latency was calculated by subtracting the pre-administration response latency from that observed post-administration.
Dexmedetomidine and levobupivacaine co-loaded, transcriptional transactivator peptide modified nanostructured lipid carriers or lipid–polymer hybrid nanoparticles, which performed better for local anesthetic therapy?
Published in Drug Delivery, 2020
Min Li, Shuo Feng, Huaixin Xing, Yingui Sun
In vivo anesthesia antinociception ability of NLCs and LPNs was assessed in rats using the hot-plate test (Singh et al., 2018). After local application of T-L&D-N, L&D-N, T-L&D-L, L&D-L, and L&D (containing 1 mg LEV and 1 mg DEX each) on the planta, rats were placed rats on a hot plate (50 °C). Response time for observed behavioral changes like paw licking, stomping, jumping, and escaping from the hot plate was recorded to examine the normal heat pain threshold before treatment and the pain threshold after treatment. The enhanced pain threshold (EPT) could be recorded using the equation: EPT (%)=(Tafter treatment – Tbefore treatment)/Tbefore treatment×100, where Tafter treatment refers to the time of threshold after treatment and Tbefore treatment is the time of threshold before treatment. The cutoff time for the hot plate test was 15 s.
Enhanced sciatic nerve regeneration by human endometrial stem cells in an electrospun poly (ε-caprolactone)/collagen/NBG nerve conduit in rat
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2018
Forouzan Mohamadi, Somayeh Ebrahimi-Barough, Mohammad Reza Nourani, Korosh Mansoori, Majid Salehi, Ali Akbar Alizadeh, Seyed Mohammad Tavangar, Farshid Sefat, Siavash Sharifi, Jafar Ai
The hot plate test is usually used for evaluating thermal pain sensitivity. During the experiment, the rats were placed in the centre of an open-ended cylindrical space with a floor consisting of a heated plate. The plate warmed to 56 °C generate two behavioural components that can be measured in terms of their reaction times, namely, paw licking and jumping. Both are considered to be integrated responses. The time elapsed from the onset of hot plate contact to a withdrawal of hind limb was measured by a timer activated by an external foot switch and recorded as withdrawal reflex latency (WRL). Cut off time was 12 s. Jumping responses were obtained at 4, 8 and 12 weeks after injury. The affected right limbs were tested three times, with an interval of 10 min between consecutive tests to prevent sensitization and the two latencies were averaged to get a final result.