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Effect of nutritional supplements on bio-plastics (PHB) production utilizing sugar refinery waste with potential application in food packaging
Published in Preparative Biochemistry and Biotechnology, 2019
Abhishek Dutt Tripathi, Tek Raj Joshi, Suresh Kumar Srivastava, Kinaoush Khosravi Darani, Shankar Khade, Jyoti Srivastava
The average molecular weight (Mn) and Polydispersity index (Mw/Mn) of the extracted polymer by sodium hypochlorite (30.0% v/v) and chloroform dispersion were determined by GPC (gel permeation chromatography), using a pump (Wayers 715 Ultra WISP, Canada), a differential refractometer detector at 30 °C (Waters 410, Canada), and a 60 cm PL gel 5 L styragel column with a linear range of molecular weight of 200–2,000,000 mol/g at 25 °C. Monodisperse polystyrene was used as the molecular weight standard. A 100 mL portion of a 0.1% w/v PHB solution was injected for each analysis. Chloroform was used as the mobile phase at a flow rate of 1.0 mL/min.