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Fluorescent Technology in the Assessment of Metabolic Disorders in Diabetes
Published in Andrey V. Dunaev, Valery V. Tuchin, Biomedical Photonics for Diabetes Research, 2023
Elena V. Zharkikh, Viktor V. Dremin, Andrey V. Dunaev
Noninvasive optical methods are increasingly used in biomedical diagnostics. Fluorescence spectroscopy, in particular, has found its application in chemistry, biology, and various fields of medicine. This method is highly sensitive and allows us to study various pathological changes of biological tissues in the development of socially significant diseases. Fluorescence spectroscopy and imaging techniques are probably the most common biomedical photonics methods used in skin research. By analyzing fluorescence data, one can extract information about the structure and component composition of the biological tissue and its functional state. Fluorescence provides insight into both the conformation of fluorescent molecules and their binding as well as their interactions within biological tissues.
Ultraviolet Electromagnetic Radiation
Published in Dave Birtalan, William Nunley, Optoelectronics, 2018
The medical analytical instrument market also utilizes UV light sources in fluorescence spectroscopy and ultraviolet-visible spectroscopy. Fluorescence spectroscopy is a type of electromagnetic spectroscopy that analyzes the fluorescence emitted from a sample being irradiated and evaluated. The light source is generally UV to excite the electrons in the specimen to emit light of a lower energy level, usually in the visible spectrum. In fluorescence spectroscopy, the sample is excited, by absorbing the higher-energy UV light, causing the sample to move from its ground electronic state to one of the various vibrational states in the excited electronic state. Analysis of the emission spectrum will permit the identification of the substance (chemical compound, food processing, cancer tumor, etc.). Fluorescence spectroscopy is also used in forensics and chemical research fields. Ultraviolet-visible spectroscopy (UV/VIS) uses multiple wavelengths of light in the visible, ultraviolet, and near-infrared ranges. The absorbance of light in a solution is directly proportional to the solution’s concentration (Beer–Lambert law).
Autofluorescence-Guided Resection of Intracranial Tumor
Published in Yu Chen, Babak Kateb, Neurophotonics and Brain Mapping, 2017
Fartash Vasefi, Zhaojun Nie, David S. Kittle, Chirag G. Patil, Pramod Butte
Fluorescence spectroscopy can be used either to investigate the variation in the intensity of fluorescence emissions (steady-state) or to detect the fluorescence decay (time-resolved). Both steady-state fluorescence spectroscopy and TRFS techniques have been investigated for their potential as an intraoperative diagnostic tool. Steady-state fluorescence spectroscopy measures the fluorescence intensity in a broadband spectral region, whereas TRFS measures the fluorescence decay or fluorescence dynamics, in addition to the intensity measurement. TRFS is considered a more robust tool for optical biopsy, as the fluorescence lifetime is independent of the emission and quantum yield, but is sensitive to changes in the tumor microenvironment. This property can be used to differentiate the fluorescence components with overlapped fluorescence emission spectra. In other words, it becomes a sensitive tool to probe the changes in microenvironment (e.g., pH, ionization, and temperature). Thus, TRFS can provide additional information compared to steady-state fluorescence techniques.
Investigating variations of fluorescent dissolved organic matter in wastewater treatment using synchronous fluorescence spectroscopy combined with principal component analysis and two-dimensional correlation
Published in Environmental Technology, 2018
Hongwei Pan, Huibin Yu, Yanan Wang, Ruixia Liu, Hongjun Lei
Fluorescence spectroscopy is showing great promise as an analytical tool for interpreting DOM fluorescence properties, although DOM includes organic molecules with chromophoric (light absorbing) and fluorophoric (light emitting) moieties [16]. The prominent advantage of fluorescence spectroscopy is that the information of DOM characteristics can be mostly obtained with rapid measurement, high sensitivity, non-destructiveness and low cost. Several fluorescence components of DOM can be determined using their fluorescence excitation–emission matrices coupled with parallel factor analysis, self-organizing map or regional integration [11,17–20].
Dry aerosol particle deposition on indoor surfaces: Review of direct measurement techniques
Published in Aerosol Science and Technology, 2022
Fluorescence spectroscopy is based on excitation of fluorescent molecules, which consequently emit fluorescent light – corresponding to a specific wavelength – captured by a detector. By using a simplified version of the Beer-Lambert law, the tracer volume concentration (g.l−1) in a solution is linked to the fluorescence emission intensity