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Published in Maik W. Jornitz, Theodore H. Meltzer, Sterile Filtration, 2020
Maik W. Jornitz, Theodore H. Meltzer
Brevundimonas diminuta, a nonpathogenic organism reclassified from Pseudomonas diminuta by Segers et al. (1994), was used to validate the 0.2/0.22 μm rated filter. It bears the American Type Culture Collection accession number 19146. Its choice as the organism for sterile filtrations stemmed from Leahy and Sullivan’s (1978) description of its desirable attributes. “It was originally isolated from contaminated solutions after filtration. Under properly controlled cultivation conditions, the cells are small and are arranged singly. In addition the organism is easily maintained and can be grown to a high cell density in a short time.” No claim was made that B. diminuta represented the smallest organism present in pharmaceutical preparations. However, by modeling the sterile filtrations afforded by the 0.2/0.22 μm rated filters, it may have seemed to many to be such, occasional failures being imputed to other causes.
Application of Genetic Engineering to the Field of Bioremediation
Published in Donald L. Wise, Debra J. Trantolo, Remediation of Hazardous Waste Contaminated Soils, 2018
Alan R. Harker, Youngjun Kim, Udayakumar Matrubutham
Nucleotide sequences were determined by the dideoxy chain termination method53 using [35S]-dATP. Taq DNA polymerase and deaza ddNTPs were used in the sequencing reactions according to the manufacturer’s recommendations. Both M13 reverse and forward primers were used. Clones used in sequencing were generated as given in Figure 1(b). Plasmid templates were prepared54,55 and layered two times on cesium chloride density gradient. Electrolyte gradient gels (6-7% with 7M urea) were used for running samples as described by Sheen and Seed.56 The nucleotide sequence has been deposited in GENBANK under the accession number M98445. The 1438-bp nucleotide sequence derived from this procedure includes the 1275-bp BamHl-Xhol, suggested to contain tfdR and complement pRO103 when expressed in trans.25 Computer analyses of the sequence reveal that the 645-bp BamHI-XbaI region is identical to the sequence upstream of tfdA in the BamHI-B fragment of pJP4. The regulatory gene tfdS has already been located in this region of BamHI-B and found to code divergently from tfdA.25,23 There are apparent duplicates of this 645-bp sequence in BamHI-H and BamHI-E fragments. Interestingly, these repeat sequences are oriented in the opposite direction to each other on the plasmid pJP4.
Informatics For Sciences: A Novel Approach
Published in Alexander V. Vakhrushev, Omari V. Mukbaniani, Heru Susanto, Chemical Technology and Informatics in Chemistry with Applications, 2019
Heru Susanto, Ching Kang Chen, Teuku Beuna Bardant
The database is seen as major tool for storing biological data for public use. Relational database concept of computer science and information retrieval concept of digital libraries is implemented to fully interpret biological database. Gene sequence, attributes, textual descriptions, and ontology classification are stored in the biological database. The data mentions are categorized as semi-structured data that later can be displayed in tables form, key delimited record, and XML structure. The common method of cross-referencing is often used by database accession number.
Photocatalytic degradation of low-density polythene using protein-coated titania nanoparticles and Lactobacillus plantarum
Published in Environmental Technology, 2023
Divyeshkumar Dave, Kamlesh Chauhan, Ankurkumar Khimani, Krina Soni, Yati Vaidya
The current study aimed to degrade the low-density plastic strips using microbes along with titania NPs isolated from damp site soil samples. Various bacterial strains were isolated. Among them, only two predominant isolated bacterial colonies were selected and used for further study (Figure 2). Two isolates PD1 & PD5 which ability to use polythene as a carbon source were used for further screening which survived in presence of 5% titania nanoparticles. Out of which PD1 isolates showed the best survival rate in presence of 5% titania nanoparticles (Figure 3). Further, these isolates were identified as Lactobacillus plantarum using DNA sequencing. And sequence submitted to the Genbank. And obtain NCBI accession number MT477833.
Screening, optimization and characterization of poly hydroxy butyrate from fresh water microalgal isolates
Published in International Journal of Biobased Plastics, 2021
Kanaga Selvaraj, Nandhini Vishvanathan, Ramamurthy Dhandapani
Screening of PHB production was done using Nile red-staining method on both Chlorella sp. and Neochloris sp. The Chlorella sp. showed better PHB accumulation Plate 11 (a). Based on the morphological features and the intensity of fluorescence maximum producers of PHB were screened. The microalgae Chlorella sp were identified as Chlorella vulgaris by molecular identification. (GenBank accession number - MZ045839).
Phosphate rock solubilization and the potential for lead immobilization by a phosphate-solubilizing bacterium (Pseudomonas sp.)
Published in Journal of Environmental Science and Health, Part A, 2020
Qi Wang, Chunqiao Xiao, Bo Feng, Ruan Chi
The 16S rRNA gene sequence of LA most closely matched that of Pseudomonas sp. with an identity of 100%. Its phylogenetic tree was constructed (Figure 2). Based on its cell morphology, physiology, and phylogenetic analysis, it was classified as Pseudomonas sp. The sequence was deposited in the GenBank nucleotide sequence data library under the accession number MK629320.