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Bio-Optical Characteristics in Relation to Phytoplankton Composition and Productivity in a Twin Arctic Fjord Ecosystem during Summer
Published in Neloy Khare, Climate Change in the Arctic, 2022
For analysis of other phytoplankton pigments, 3 L of seawater was filtered onto GF/F filters (0.7 μm, 25 mm, Whatman®) in a dark and cold room, and stored at −80°C until further analysis. For logistical limitation, samples for high-performance liquid chromatography (HPLC) analysis were collected for phase 1 only. The preserved filters were soaked in 100% methanol for extraction of pigments and subsequently analysed by a HPLC (Agilent Technologies) equipped with an Eclipse XDB C8 column as detailed in Kurian et al. (2012). For phytoplankton taxa classification (Table 13.2), seven pigments such as fucoxanthin, peridinin, alloxanthin, 19′-hexanoyloxyfucoxanthin (19′HF), 19′-butanoyloxyfucoxanthin (19′BF), zeaxanthin and TChl-b (Chl-b+divinyl chlorophyll-b (DivChl-b)) were identified as diagnostic pigments (DP) according to Uitz et al. (2006). Using DP, size-fractioned (microphytoplankton (>20 µm), nanophytoplankton (>2 and <20 µm) and picoplankton (<2 µm)) contributions of phytoplankton (fmicro, fnano, and fpico) were derived as follows:
Triplet-state spin labels for highly sensitive pulsed dipolar spectroscopy
Published in Molecular Physics, 2019
M. G. Dal Farra, S. Ciuti, M. Gobbo, D. Carbonera, M. Di Valentin
PCP is the peripheral, water soluble light-harvesting complex of dinoflagellate algae, containing only chlorophyll a and the carotenoid named peridinin in a ratio of 1:4. The N- and C-terminal domains of the monomers are related by a pseudo-twofold symmetry and each of them contains a cluster of pigments with the central chlorophyll a surrounded by four peridinin molecules [30]. The protein arrangement is reported in Figure 4.