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Ion-Exchange Processes
Published in Juan A. Asenjo, Separation Processes in Biotechnology, 2020
Since a bed of ion-exchange resin serves as a good filter, a fermentation broth must be filtered or centrifuged before being applied to the resin to prevent plugging of the column with insoluble by-products. Some antibiotic is inevitably lost during those steps. To maximize the antibiotic yield and minimize equipment expense, a process to exchange the antibiotic directly from the unfiltered broth was developed by Bartels in 1957 (Belter, 1984). Even though there are few literature references discussing commercial antibiotic processes, Belter et al. (1973) have published a detailed description of this type of process used for the isolation of novobiocin from an unfiltered broth.
Downstream Processing Extraction of Fermentation Products
Published in Nduka Okafor, Benedict C. Okeke, Modern Industrial Microbiology and Biotechnology, 2017
Nduka Okafor, Benedict C. Okeke
In some fermentations such as the acetone-butanol fermentation, the whole unseparated broth is stripped of its content of the required product. In the antibiotic industry, a similar situation was achieved before it became possible to directly absorb the antibiotics streptomycin (using cationic-exchange resin) and novobiocin (on an anionic resin). The antibiotics are eluted from the resins and then crystallized. This process saves the capital and recurrent expense of the initial separation of solids from the broth.
Antimicrobial activities of amphiphilic cationic polymers and their efficacy of combination with novobiocin
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Atsushi Miyagawa, Shinya Ohno, Tomohiko Hattori, Hatsuo Yamamura
In this study, novobiocin was selected as the antibiotic for combined use with antimicrobial polymers. The antimicrobial mechanism of novobiocin is that it acts as a DNA synthesis inhibitor. Novobiocin shows high antimicrobial activity against gram-positive bacteria, which have a thick peptidoglycan layer, but not against gram-negative bacteria, which have an outer membrane and thin peptidoglycan layer [46,47]; novobiocin cannot permeate this outer membrane. The minimum inhibitory concentration (MIC) of novobiocin against the gram-negative bacterium E. coli is 128 μg/mL and that against the gram-positive bacterium S. aureus is 1 μg/mL. If novobiocin can penetrate E. coli through the outer membrane, it shows antimicrobial activity [48–51].